A cell line derived from a malignant hamster tumor was made resistant to 1/ag/ml actinomycin D by continuous exposure to increasing concentrations of the antibiotic. In addition to a normal nucleolus, the resistant cells exhibited several small exhausted ones in the terminal phase of nucleolar segregation. Comparative growth rate and incorporation of uridine-3H revealed a significant delay in t...

metabolites. The Chemical Society, Special Publication No. 5, London, England. KOFFLER, H., EmERSON, R. L., PERLMAN, D., AND BURRIS, R. H. 1945 Chemical changes in submerged penicillin fermentations. J. Bacteriol., 60, 517-548. O'BRIEN, E., WAGMAN, G. H., AND PERLMAN, D. 1952 Synthetic media for growth and streptomycin production by Streptomyces griseus. Bacteriol. Proc., p. 25. PUGH, L., KATZ,...

The toxic effects of the combinations of amphotericin B (AmB) and actinomycin D or AmB and 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea were measured against the human promyelocytic leukemia cells HL-60. The toxicities of both drug combinations were greater than the additive toxicity of each of the drugs used singly, but the exact conditions under which synergism was achieved differed for each ...

Chinese hamster cell lines resistant to daunomycin (DM) were developed by direct exposure to the antibiotic. Increase in resistance up to 883-fold was obtained. Evaluation of dose-response data for sensitive and resistant sublines revealed complete cross-resistance to the chemically closely related antibiotic adriamycin as well as considerable cross-resistance to actinomycin D. Radioautographic...

Molecular models of the complexes between actinomycin D and 14 different DNA hexamers were built based on the X-ray crystal structure of the actinomycin-d(GAAGCTTC)2 complex. The DNA sequences included the canonical GpC binding step flanked by different base pairs, nonclassical binding sites such as GpG and GpT, and sites containing 2,6-diamino-purine. A good correlation was found between the i...

Demonstrationi of a necessary role for RNA and protein synthesis in maintenance of rapid rates of cell expansion in planit tissues has been greatly facilitated through use of actinomycin D, puromycin., 8azaguanine and other specific inhibitors (5, 6, 7, 11). For example, a low concentration of the antibiotic actinomycin D will partially inhibit protein synthesis (5, 7, 11), and at the same time...

The interactions of actinomycin D (ActD) anticancer drug with two bile salts different hydrophobicity (sodium cholate (NaC) and sodium deoxycolate (NaDC) the influence these aggregates on ActD?DNA complex was investigated in 10 mM phosphate buffer (pH 7.4) by UV?Vis spectroscopy (absorption thermal denaturation). binding strength ActD to NaDC is higher than for NaC, this difference attests stro...

Gestational trophoblastic neoplasia (GTN) is the term to describe a set of malignant placental diseases, including invasive mole, choriocarcinoma, placental site trophoblastic tumor and epithelioid trophoblastic tumor. Both invasive mole and choriocarcinoma respond well to chemotherapy, and cure rates are greater than 90%. Since the advent of chemotherapy, low-risk GTN has been treated with a s...

Six strains of gram-negative anaerobic fusiform rods (Fusobacterium and Bacteroides spp.), isolated from deep subgingival locations in humans, were examined for (i) gliding motility in slide cultures, (iii) cell densities on nutrient agar surfaces, and (iii) sensitivity to actinomycin D. Known gliding (FBt) and nonmotile (NM) strains of Myxococcus xanthus served as controls for the gliding and ...

Abstract The usefulness of adjuvant chemotherapy for high-grade osteosarcoma was established by two randomized, controlled trials conducted in the 1980s, which used six drugs, doxorubicin, cisplatin, high-dose methotrexate, bleomycin, cyclophosphamide and actinomycin D. Since then, development has been promoted direction introducing preoperative chemotherapy, changing post-operative according t...