Piper nigrum is broadly acted as a herbal medicine and anticancer activity well. Previously, we reported that extract caused induction of breast cancer cells death reduction cell migration in vitro study. In this present study, need to explore the effects on human HeLa cervical underlying mechanism. Sulforhodamine B, colony formation, cycle arrest, acridine orange/ethidium bromide staining was ...

In this study, 8 novel pyrazole disperse dyes which were synthesized in our previous work [1] applied to polyethylene terephthalate (PET), poly (lactic acid) (PLA) and polyamide 6.6 (PA 6.6) fiber fabrics their colorimetric fastness properties evaluated. The measured a*, b* hue angle (ho) values of all dyed exhibited yellow, orange red colour shades. Quite high strength (K/S) particularly for c...

Most traditional anticancer medications are ineffective in treating triple negative breast cancer (TNBC), which has enhanced resistance to chemotherapeutic agents. Herbs continue be a key element the discovery of medications. In present study, we assessed cytotoxicity methanol extract leaves Pergularia daemia (MLPD) MDA-MB-231 triple-negative (TNBC) cell line. The MLPD was using MTT assay revea...

Treatment of cultured pancreatic B-cells (HIT-T15 and RINm5F) with the diabetogenic drug streptozotocin resulted in a significant increase in the number of cells that became detached from the substrate during a subsequent culture period. Examination of the detached cells by fluorescence microscopy after staining with acridine orange or by electron microscopy revealed evidence of chromatin conde...

Mouse peritoneal macrophages exposed to type-specific polysaccharide and peptidoglycan (PPG) from group A streptococci undergo marked morphologic and biochemical changes. The cells show increases in size and an increased number of lysosomes as demonstrated by vital staining with acridine orange. There are significant elevations in the levels of both lysosomal and nonlysosomal enzymes. Higher do...

The DNA content of individual plastids of the giant unicellular algae Acetabularia mediterranea, and Polyphysa cliftoni was studied. Four methods were used for localizing DNA: acridine orange staining, radioautography following actinomycin D-(3)H treatment, electron microscopy of thin tissue sections, and electron microscopy of osomotically disrupted plastids. With each method, DNA was readily ...