Gangliosides and sulfatides (STs) are acidic glycosphingolipids (GSLs) that have one or more sialic acids or sulfate substituents, in addition to neutral sugars, attached to the C-1 hydroxyl group of the ceramide long chain base. TLC is a widely employed and convenient technique for separation and characterization of GSLs. When TLC is directly coupled to MS, it provides both the molecular mass ...

SUMMARY The saline-soluble proteins of rat liver nuclei have been cx tensively resolved into profiles according to charge, permitting comparison with similar arrays from liver cytoplasm. Nuclei were isolated in 2.1 M sucrose solution from the per fused livers of rats fed stock, control, or hepatocarcinogenic 3'-methyl-4-dimethylaminoazobenzene diet. After feeding the 320 dye for 20 days, the nu...

A simple and sensitive spectrophotometric method was developed for the determination of trace amounts of sulfur dioxide. The method is based on the reaction of SO2 with a known excess of ICI as the oxidant. The unreacted ICI iodinates thymol blue under acidic conditions. The lambdamax of thymol blue is at 545 nm under acidic conditions, and on lodination lambdamax shifts to 430 nm. This shift r...

Human prothymosin alpha (ProTalpha) is a small acidic protein (12.1 kDa; pI approximately 3.5) ubiquitously expressed in a wide variety of tissues. The amino acid composition of this protein is highly unusual. While close to half of its sequence is composed of acidic amino acids, the protein does not contain any aromatic residues. ProTalpha has been shown to play crucial roles in different biol...

The objective of this paper was to investigate the molecular characterization soil organic matter fractions (humic substances (HS): fulvic acids-FAs, humic acids-HAs, and humins-HNs), which are most reactive components. A wide spectrum spectroscopic (UV–VIS VIS–nearIR), as well electrochemical (zeta potential, particle size diameter, polydispersity index), methods were applied find relevant dif...

The Herman Pit, once a mercury mine, is an impoundment located in an active geothermal area. Its acidic waters are permeated by hundreds of gas seeps. One seep was sampled and found to be composed of mostly CO₂ with some CH₄ present. The δ(13)CH₄ value suggested a complex origin for the methane: i.e., a thermogenic component plus a biological methanogenic portion. The relatively (12)C-enriched ...

In the present work the electrodialytic remediation technique is applied for the treatment of fly ash, a hazardous by-product resulting from the incineration of municipal solid waste. Results are presented for an experiment conducted for 40 days at 38 mA, with a continuously stirred cell. Experimental parameters monitored include voltage drop, pH and electrolyte’s volumes. Evolution of heavy me...

A survey of the experimental equilibrium constants in solution for mixed complexes 4f ions with acidic (chelating) and O-donor organophosphorus ligands published period between 1954 2022 is presented. These data are widely used both analytical solvent extraction chemistry. Important evaluation criteria involved specification essential reactions, process conditions correctness techniques calcula...

UNLABELLED Lactate is a key myocardial energy source. Lactate metabolism is altered in a variety of conditions, such as exercise and diabetes mellitus. However, to our knowledge, noninvasive quantitative measurements of myocardial lactate metabolism have never been performed because of the lack of an adequate radiotracer. In this study we tested L-3-(11)C-lactate ((11)C-lactate) as such a trace...