2,4-Dichlorophenoxyacetic acid (2,4-D) is an herbicide that was one of the two active ingredients in Agent Orange, the Vietnam War defoliant. Although the main health effects of Agent Orange were blamed on the other component of the mixture (2,4,5-T) and dioxin contamination, the data indicate that 2,4-D has significant health risks of its own. It remains unclear whether continuing low-level di...

Multiwalled carbon nanotubes were used to modify the surface of a glassy carbon electrode to enhance its electroactivity. Nafion served to immobilise the carbon nanotubes on the electrode surface. The modified electrode was used to develop an analytical method for the analysis of ascorbic acid (AA) by square-wave voltammetry (SWV). The oxidation of ascorbic acid at the modified glassy carbon el...

Settings. National Institute for Research in Tuberculosis, Chennai. Objective. To assess the proportion of metabolically active cells of Mycobacterium tuberculosis after exposed to CPC using FDA-EB vital staining and viable counts on LJ medium. Mycolic acid content in M. tuberculosis after exposure to CPC was estimated using HPLC. Methods. Clinical isolates of M. tuberculosis and standard refer...

'The uptake of vital dyes by lysosomes (1, 2) has permitted the microscopic observation of these subcellular organelles in living cells (3) and tissue homogenates (4) . Acridine orange (3 , 6-bis-dimethylaminoacridine), a fluorescent metachromatic dye, has been shown to concentrate within lysosomes, causing them to appear as bright orange granules in fluorescence microscopy (5-7) . We have obse...

Vacuolar pyrophosphatase (V-PPase) from juice cells of 3 citrus varieties (differing in their vacuolar pH) were partially characterized using purified tonoplast vesicles. Total V-PPase activity was highest in vesicle samples from sweet limes with vacuolar pH of 5.0, while samples from acid limes (with lowest vacuolar pH of 2.0) had the minimal total V-PPase activity. Samples from 'Valencia' ora...