Human cryptosporidiosis is an infection caused by Cryptosporidium spp, a coccidial protozoan of emergencial pathogenicity and responsible for severe and prostrating watery diarrhea, mainly in immunocompromised patients. Smears of stools submitted to concentration and staining by carbol fuchsin technique has been used in our laboratory as a diagnostic procedure for cryptosporidiosis. The long ti...

background: actinomycosis is an indolent, slowly progressive infection caused by non acid fast, gram positive, anaerobic or microaerophilic bacteria. the most common site of actinomycosis infection is cervicofacial region. bacteriologic identification from infected site or detection of sulfur granules confirms the diagnosis. we report a rare case of simultaneous infection of larynx and lungs ac...

Sputum microscopy is the most important tool for detection of Mycobacterium tuberculosis in peripheral laboratories. Sodium hypochlorite concentration technique prior to sputum may improve AFB bacilli over direct Ziehl Neelsen’s staining method. A prospective study was conducted at Kasturba Medical College, Manipal. Consecutive samples were collected 3 months. ZN stained smears made directly fr...

Tuberculosis in acute myeloid leukemia (AML) has rarely been reported. Herein, we report the diagnosis and treatment of a patient with AML who was finally diagnosed pulmonary tuberculosis following consolidation chemotherapy. In this case, chest CT showed space-occupying lesions near right hilum after second cycle Initially, extramedullary infiltration lung cancer were considered. After chemoth...

Introduction: Lichenoid granulomatous dermatitis (LGD) is widely encountered lesions with both oral as well dermal manifestation. Present study was done to evaluate lichenoid stomatitis cases. Materials and methods: 226 biopsies were exposed special stains such acid-fast bacilli (AFB), immunohistochemical staining for CD 68 Grocott methenamine-silver (GMS), periodic acid‐Schiff (PAS) stains. Re...

OBJECTIVE To search for the opportunistic and other pathogenic intestinal parasites in dialysis patients, and to compare the methods used for diagnosis. METHODS This is a randomized study, which recruited participants from the dialysis patients. The study was carried out in the Department of Microbiology, Research Hospital, School of Medicine in Kocaeli University, Kocaeli, Turkey between Jun...

Studies seeking Mycobacterium avium subsp. paratuberculosis in Crohn's disease by PCR have generated inconsistent findings. As an alternative, microscopy offers a number of advantages, including direct visualization of organisms in tissue. Experimental infections have demonstrated that M. avium organisms can be seen by both acid-fast staining and species-specific in situ hybridization, but beca...

Coccidian protozoa of Cyclospora cayetanensis are obligate intracellular apicomplexan parasites that infect the mucosal epithelium of the small intestine of immunocompetent and immunocompromised persons. A 25- years old woman from around, Tehran with complaint of faintness and fatigue with HIV positive/AIDS confirmed eight months ago was admitted in Imam Khomeini Hospital, Tehran, Iran in 2014....

The aims of this study were to determine the prevalence of Cryptosporidium spp in dairy cows in central Thailand and to investigate the genotype of Cryptosporidium spp in this population. A total of 200 fecal samples from dairy cows were collected and examined by the acid-fast staining technique and polymerase chain reaction restriction fragment length polymorphism (PCR-RFLP). The prevalence of...