نتایج جستجو برای: acid dissociation constant
تعداد نتایج: 982380 فیلتر نتایج به سال:
Background and Aim: The affinity of an antibody with its antigen is a crucial parameter in its biological activity and carrying out immunologic tests such as radioimmunoassay (RIA) and immunohistochemistry. This study was done in our laboratory at Tarbiat Modarres University to measure the affinity constant of specific monoclonal antibodies/MAB (A1G8F7) with alkaline phosphatase enzyme produced...
The precipitation of strontium in highly concentrated nitric acid is often used standard radiochemical methods for 90Sr separation. In this work, the description Sr behavior such conditions was studied point speciation simulation. Standard method based on Phreeqc software considered but two problems underlined limits approach to study process: inappropriate ionic strength correction model (for ...
Solar cells based on organometal halide perovskites have seen rapidly increasing efficiencies, now exceeding 15%. Despite this progress, there is still limited knowledge on the fundamental photophysics. Here we use microwave photoconductance and photoluminescence measurements to investigate the temperature dependence of the carrier generation, mobility, and recombination in (CH3NH3)PbI3. At tem...
The iron-dependent regulator (IdeR) is a 230-amino acid transcriptional repressor that regulates iron homeostasis, oxidative stress response and virulence in Mycobacterium tuberculosis. The natural ligand for IdeR is Fe(II), but Ni(II), Co(II), Cd(II), Mn(II), and Zn(II) also bind to and activate the protein in vitro. Protein activation by metal is a complex process involving metal-induced fold...
Hemoglobin I was isolated from nodules formed on the roots of Parasponia andersonii inoculated with Rhizobium strain CP 283. The rate of oxygen dissociation from Parasponia hemoglobin increases about 12-fold between pH 4 and 7, with apparent pK 6.4, to reach a limiting value of 14.8s-1. The optical spectrum of oxyhemoglobin in the visible region is also dependent on pH with pK near 6.4. The rat...
We have studied the binding of alpha-bungarotoxin to a particulate fraction of goldfish brain enriched in synaptosomes. The binding is specific and saturable and exhibits the pharmacological properties of a nicotinic cholinergic receptor. Equilibrium binding measurements yield a single dissociation constant (KD) of 0.92 nM. Kinetic analysis revealed one association rate constant and two dissoci...
The j3 nerve growth factor (NGF) isolated from the high molecular weight 7 S NGF complex from mouse submaxillary gland is a protein of molecular weight 26,500 which contains two noncovalently associated peptide chains. A variety of techniques were employed to determine whether the NGF dimer dissociates at the low concentrations at which it is biologically active. No dissociation was detected in...
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