An enormous number of alternative pre-mRNA splicing patterns in multicellular organisms are coordinately defined by a limited number of regulatory proteins and cis elements. Mutually exclusive alternative splicing should be strictly regulated and is a challenging model for elucidating regulation mechanisms. Here we provide models of the regulation of two sets of mutually exclusive exons, 4a-4c ...

This paper describes stereospecific side chain hydroxylations in the biosynthesis of chenodeoxycholic acid by liver mitochondria and microsomes. The hydroxylation of 5/3-cholestane-3a,7a-diol was studied in subcellular fractions of rat, guinea pig, and rabbit livers. Incubation of SD-cholestane-3a,7n-diol resulted in hydroxylations at C-12, C-24, C-25, and C-26. The biosynthetic bile alcohols w...

A simple and rapid enzymic fluorimetric method for the determination of sulphated and non-sulphated primary bile acids in urine has been developed. Octadecyisilane-bonded silica cartridges (Sep-Pak CI8) are used for the solid-phase extraction of bile acids (BA) from urine samples. Sulphated BA are solvolysed before measurement with an improved rapid solvolysis procedure. The measurement is base...

Menin, a protein encoded by the MEN1 gene, is mutated in patients with multiple endocrine neoplasia type 1 (MEN1). Menin acts as a tumor suppressor in endocrine organs while it is also required for transformation of a subgroup of leukemia. The recently solved crystal structure of menin with different binding partners reveals that menin is a key scaffold protein that cross-talks with various par...

1. The metabolism of 4-[4-14C]androstene-3,17-dione, 4-[4-14C]pregnene-3,20-dione, 5a-[4-14C]androstane-3a,17f8-diol, [4-4C]cholesterol, 7a-hydroxy4-6fi-3H]cholesten3-one, 5fi-[7fi-3H]cholestane-3a,7U-diol and [3H]lithocholic acid was studied in the microsomal fraction of livers from control and orotic acid-treated male rats. 2. As a result of the treatment the orotic acid-fed rats had fatty li...

We analysed spatio-temporal expression of dorso-ventral genes - Wnt-7a, En-1, Lmx-1 and Fgf-8 - during both normal and ectopic limb formation following fibroblast growth factor (FGF) application to the flank. We confirm that Wnt-7a is the first of these genes to be expressed in dorsal ectoderm in limb-forming regions. We also noticed patterns and kinetics of gene expression specific to chick th...

The purpose of this study was to determine whether the BACTEC NR-16A and NR-17A media were more effective than the BACTEC NR-6A and NR-7A media in recovering organisms from the blood of patients undergoing antimicrobial therapy. A total of 986 sets of four blood culture bottles were compared, giving 141, 174, 93, and 104 isolates with BACTEC NR-6A, NR-16A, NR-7A, and NR-17A, respectively. BACTE...

Corrosion resistance of Gold 21K alloy immersed in artificial sweat the absence and presence 100 ppm urea also D-Glucose has been investigated by polarization study AC impedance spectra. It is observed that / increases. Hence it concluded people wearing ornaments made need not worry about excess their sweat. When urea, Linear Polarisation Resistance value increases from 103389Ohmcm2 to 123437Oh...

to locate the genes controlling adaptability in bread wheat using ammi (additive main effect and multiplicative interaction) model, twenty-one substitution lines derived from the parents chinese spring (recipient) and chayan (donor) were used in a randomized complete block design with three replications in three different environments in the agricultural college of razi university, kermanshah, ...