Serum vitamin B,2 levels of 2523 individuals were measured using the hemoglobin-coated charcoal assay. In one hespital, the test was performed on 1698 patients under hematologic evaluation for erythrocytic abnormalities of all types, while 825 patients from five other hospitals were studied because of suspected B,2 deficiency. The incidence of low serum vitamin B,2 was surprisingly low for both...

Serum vitamin B,2 levels of 2523 individuals were measured using the hemoglobin-coated charcoal assay. In one hespital, the test was performed on 1698 patients under hematologic evaluation for erythrocytic abnormalities of all types, while 825 patients from five other hospitals were studied because of suspected B,2 deficiency. The incidence of low serum vitamin B,2 was surprisingly low for both...

OBJECTIVE we conducted a case-control study to investigate the association between dietary folate, vitamin B6 and vitamin B12 intake, MTHFR and MTR genotype, and breast cancer risk. METHODS Genotyping for MTHFR C677T and A1298C and MTR A2756G polymorphisms were performed using polymerase chain reaction-restriction fragment length polymorphism analysis (PCR-RFLP) method. The intake of folate, ...

Serum vitamin B,2 levels of 2523 individuals were measured using the hemoglobin-coated charcoal assay. In one hespital, the test was performed on 1698 patients under hematologic evaluation for erythrocytic abnormalities of all types, while 825 patients from five other hospitals were studied because of suspected B,2 deficiency. The incidence of low serum vitamin B,2 was surprisingly low for both...

The importance of toll-like receptor 4 (TLR4) in immunity to rickettsiae remains elusive. To investigate the role of TLR4 in protection against rickettsioses, we utilized C3H/HeJ mice, which are naturally defective in TLR4 signaling, and compared the responses of C3H/HeN and C3H/HeJ mice following intravenous inoculation with Rickettsia conorii. Mice genetically defective in TLR4 signaling deve...

Cytochrome P450(eryF) (CYP107A1), which hydroxylates deoxyerythronolide B in erythromycin biosynthesis, lacks the otherwise highly conserved threonine that is thought to promote O-O bond scission. The role of this threonine is satisfied in P450(eryF) by a substrate hydroxyl group, making deoxyerythronolide B the only acceptable substrate. As shown here, replacement of Ala(245) by a threonine en...