The pseudorabies virus (PRV) DNase gene has an open reading frame of 1476 nt, capable of coding a 492-residue protein. A previous study showed that PRV DNase is an alkaline exonuclease and endonuclease, exhibiting an Escherichia coli RecBCD-like catalytic function. To analyse its catalytic mechanism further, we constructed a set of clones truncated at the N-terminus or C-terminus of PRV DNase. ...

A complement of cone visual pigments was identified in the Japanese anchovy Engraulis japonicus, one of the engraulid fish species that has a retina specialized for polarization and color vision. The nature of the chromophore bound to opsin proteins was investigated using high performance liquid chromatography. The opsin genes were then cloned and sequenced, and the absorption spectra of differ...

It has been demonstrated that HLA-B*5701 screening reduces the risk for hypersensitivity reaction to abacavir in HIV-infected patients. Since B*5701 prevalence varies among different populations, it is important to determine the carrier frequency prior to its use for the screening of HIV-infected patients.The aim of this study was to determine HLA-B*5701 carrier frequency in Chilean general pop...

487 HISTORY OF INTERVIEWING 488 PURPOSE OF ASSESSMENT INTERVIEWS 488 TYPES OF INTERVIEWS 488 Case History Interviews 489 Diagnostic Interviews 489 Follow-Up Interviews 489 Forensic Interviews 489 Intake Interviews 490 Interviewing for Etiology 490 Mental Status Exams 490 Orientation Interviews 490 Preand Posttesting Interviews 490 Screening Interviews 491 Specialized Interviews 491 Termination ...

BACKGROUND We study the human serum albumin (HSA) protein-CuO nanoparticle interaction to identify the specific binding site of protein with CuO nanoparticles by molecular docking and compared it with HSA-TiO2 nanoparticle interaction. METHODS The protein structural data that was obtained using Autodock 4.2. RESULTS In case of CuO np-HSA interaction, the distances from the centre of Subdoma...

INTRODUCTION Patient management following elective cranial surgery often includes routine postoperative computed tomography (CT). We analyzed whether a regime of early extubation and close neurological monitoring without routine CT is safe, and compared the rate of postoperative emergency neurosurgical intervention with published data. METHODS Four hundred ninety-two patients were prospective...

1 Genetic and pharmacological inactivation of the purinergic P2RX7 receptor 2 dampens inflammation but increases tumor incidence in a mouse model of 3 colitis-associated cancer 4 5 Paul Hofman, Julien Cherfils-Vicini, Marie Bazin, Marius Ilie, Thierry Juhel, 6 Xavier Hébuterne, Eric Gilson, Annie Schmid-Alliana, Olivier Boyer, Sahil 7 Adriouch and Valérie Vouret-Craviari # 8 9 1: Institute for ...

Pseudomonas exotoxin A (PE) is a single-chain 66-kDa polypeptide that kills eukaryotic cells by ADP-ribosylation of translational elongation factor 2. PE is composed of three major structural domains whose functions are binding of cells (I), translocation (II), and ADP-ribosylation (III). Here we describe a protease cleavage target that is located near arginine-490 on the surface of domain III....

Asian stock markets are compared with European markets before and during the 1997 Asian crisis. The clinical issue is whether regional inter-dependence became larger around the crisis, fomenting investor fears of contagion and reducing asset values because of lower diversification potential. Statistical measures are developed to aid in this inquiry. We find that European and East Asian countrie...

Cell division cycle 25B is a key cell cycle regulator and widely considered as potent clinical drug target for cancers. This research focused on identifying potential compounds in theory which are able to disrupt transient interactions between CDC25B and its CDK2/Cyclin A substrate.By using the method of ZDOCK and RDOCK, the most optimized 3D structure of CDK2/Cyclin A in complex with CDC25B wa...