نتایج جستجو برای: 30 expression vector

تعداد نتایج: 1528184  

Journal: :novelty in biomedicine 0
mehdi shokri immunology department and virology department, pasteur institute of iran, tehran, iran soheila ajdary immunology department, pasteur institute of iran, tehran, iran mina ebrahimi-rad biochemistry department, pasteur institute of iran, tehran, iran arash memarnejadian hepatitis and aids department, pasteur institute of iran, tehran, iran mohamad-hossein alimohamadian immunology department, pasteur institute of iran, tehran, iran fatemeh motevali hepatitis and aids department, pasteur institute of iran, tehran, iran

background: leishmania major lmsti1 is a conserved protein among different species of leishmania, and expressed in both amastigote and promastigote forms of l. major life cycle. it has previously been expressed in bacterial systems. m aterials and methods: to express lmsti1 in the methylotrophic yeast         pichia pastoris ( p. pastoris ), the shuttle vector ppicza containing gene lmsti1 was ...

Journal: :iranian journal of biotechnology 2008
malihe nazari jahantigh kamran ghaedi mohamad hossein nasr isfahani somayeh tanhaei farzaneh rabiee

epitope tagging is a method of expressing proteins whereby an epitope for a specific monoclonal antibody is fused to a target protein using recombinant dna techniques. the aim of this study was to sub-clone the peroxisomal protein (pep) cdna into a mammalian expression vector leading to the formation of a  chimeric pep-cdna containing the flag epitope. the flag-pep recombinant cdna was construc...

Journal: :Science 2004
Benedetta Dorigo Thomas Schalch Alexandra Kulangara Sylwia Duda Rasmus R Schroeder Timothy J Richmond

Chromatin folding determines the accessibility of DNA constituting eukaryotic genomes and consequently is profoundly important in the mechanisms of nuclear processes such as gene regulation. Nucleosome arrays compact to form a 30-nanometer chromatin fiber of hitherto disputed structure. Two competing classes of models have been proposed in which nucleosomes are either arranged linearly in a one...

2017
Ekaterina V. Nizovtseva Nicolas Clauvelin Stefjord Todolli Yury S. Polikanov Olga I. Kulaeva Scott Wengrzynek Wilma K. Olson Vasily M. Studitsky

Communication between distantly spaced genomic regions is one of the key features of gene regulation in eukaryotes. Chromatin per se can stimulate efficient enhancer-promoter communication (EPC); however, the role of chromatin structure and dynamics in this process remains poorly understood. Here we show that nucleosome spacing and the presence of nucleosome-free DNA regions can modulate chroma...

H. Goudarzi H. Keyvanfar M. Kamalzade M. Lotfi M.R. Seyfi Abad Shapouri S.A. Pourbakhsh,

  With advent and development of DNA recombinant technology and advantages of p. pastoris expression system, fusion (F) protein of PPRV expression, because of effective immunodominant role could be an appropriate candidate for production of recombinant vaccine against PPR disease. In this study, F gene of PPRV Nigeria 75/1 strain (1637 bp) was amplified using RT-PCR and purified. It was then cl...

Alami Saeid Kh Dormyani K Foruzanfar M Ghaedi K Nasr Esfahani MH, Pirjamali L,

Background: The hyaluronidases are the enzymes hydrolyze β-1, 4 glycosidic linkage of hyaluronan. Hyaluronan is a polymer consisting of a repeating disaccharide unit found in cumulus ovuforus complex, semen liquid and other tissue. Addition to hydrolyzing the hyaluronan, hyaluronidase can penetrate through the cumulus cells layer that surrounds the oocyte, thus it terms spreading factor. Moreov...

Journal: :iranian journal of basic medical sciences 0
mahrou sadri blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran department of medical biotechnology, faculty of allied medicine, iran university of medical sciences, tehran, iran mohammad m farajollahi department of medical biotechnology, faculty of allied medicine, iran university of medical sciences, tehran, iran zohreh sharifi blood transfusion research center, high institute for research and education in transfusion medicine, tehran, iran

objective(s): hepatitis c is a major cause of liver failure worldwide. current therapies applied for this disease are not fully effective and produce side effects in most cases. non-structural protein 3 helicase (ns3) of hcv is one of the key enzymes in viral replication and infection. therefore, this region is a promising target to design new drugs and therapies against hcv infection. the aim ...

Mahrou Sadri Mohammad M Farajollahi Zohreh Sharifi,

Objective(s): Hepatitis C is a major cause of liver failure worldwide. Current therapies applied for this disease are not fully effective and produce side effects in most cases. Non-structural protein 3 helicase (NS3) of HCV is one of the key enzymes in viral replication and infection. Therefore, this region is a promising target to design new drugs and therapies against HCV infection. The aim ...

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