To map the DNA-binding domain of polyomavirus large T antigen, we constructed a set of plasmids coding for unidirectional carboxy- or amino-terminal deletion mutations in the large T antigen. Analysis of origin-specific DNA binding by mutant proteins expressed in Cos-1 cells revealed that the C-terminal boundary of the DNA-binding domain is at or near Glu-398. Fusion proteins of large T antigen...

Ni-base superalloys are essential materials for high-temperature applications in the energy and aerospace sectors. Significant benefits design, function, manufacture of components may be realized from additive manufacturing (AM) these materials. However, because cracking issues during AM fabrication, only a handful have been tried qualified. This article provides an initial evaluation processab...

Abstract Concentrate withdrawal and feed restriction reduce milk production to facilitate dry-off. We investigated the impact of rations providing different amounts nutrients in combination with on performance, endocrine, metabolic responses before after abrupt Forty-three cows were studied from d 12 until 6 dry-off (56 scheduled calving). Cows fed experimental concentrates rich either crude pr...

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Philosophy of Education in the Semiotics Charles Peirce. A Cosmology Learning and Loving de Alin Olteanu / OLTEANU, Loving, Oxford/Berlin, Peter Lang, 2015, 282 p.. Un article la revue Cygne noir (Sémiotique des mystères) diffusée par plateforme Érudit.