In the present study we evaluated the amount and distribution of genetic variation by using RAPD marker variation of 12 markers loci in three broodstock groups of rainbow trout. A total of 47 polymorphic bands were observed in Iranian strain, average number of bands was 10 and average number of polymorphic bands per primer was 3.92. The total detected bands in rainbow trout strain originated fr...

In the present study we evaluated the amount and distribution of genetic variation by using RAPD marker variation of 12 markers loci in three broodstock groups of rainbow trout. A total of 47 polymorphic bands were observed in Iranian strain, average number of bands was 10 and average number of polymorphic bands per primer was 3.92. The total detected bands in rainbow trout strain originated fr...

Over the last two decades, DNA fingerprinting have been increasingly used to characterize germplasm of different organisms including crop plants, which was otherwise difficult with morphological and or isozyme markers because of paucity of genetic information at phenotypic level. In the present study, two hybrid samples of sorghum (Sorghum bicolor L.) received from Federal Seed Certification an...

the genetic diversity of a large number of pistachio genotypes grown in iran is not exactly known. most of the studies on genetic diversity of iranian pistachio varieties are based on morphological characteristics or isozyme markers. in the present study, the genetic diversity of selected pistachio cultivars along with some wild species were evaluated by amplified fragment length polymorphism (...

Pleurotus eryngii, known as king oyster mushroom has been widely used for nutritional and medicinal purposes. This study was initiated to screen the suitable conditions for mycelial growth and to determine the phylogenetic relationship of the selected strains. Optimal mycelial growth was observed at 30℃ and minimum mycelial growth observed at 10℃. This mushroom tolerates a broad pH range for my...

Bacterial wilt, caused by Ralstonia solanacearum E.F. Smith, is one of the most destructive diseases of tomato (Lycopersicon esculentum Mill) in Sri Lanka. Twenty isolates of R. solanacearum from tomato plants grown in eleven different agro climatic zones from selected districts (Kandy, Matale, Monaragala) were characterized in terms of pathogenicity on susceptible check variety Marglobe and in...

A multiplex nested PCR technique was used to identify gender from single blastomeres biopsied from 8-cell mouse embryos. The primers amplified sequences of the Sry and Zfy genes on the Y-chromosome, and a polymorphic X chromosome microsatellite locus (DXNds-3). Amplification of male DNA gave three bands, of sizes 217 bp (Zfy), 147 bp (Sry) and 111 bp (Nds). In contrast, amplification of female ...

Random amplified polymorphic DNA (RAPD) with universal rice primers (URP) was used to identify species and to determine phylogenetic relationships for the 6 economically important Korean Pacific abalone species: Haliotis discus hannai, H. discus discus, H. madaka, H. gigantea, H. diversicolor supertexta, and H. diversicolor diversicolor, whose morphological differentiation is difficult. Of the ...