Background: Identification of radioisotopes for plastic scintillation detectors is challenging because their spectra have poor energy resolutions and lack photo peaks. To overcome this weakness, many researchers conducted radioisotope identification studies using machine learning algorithms; however, the effect data normalization on has not been addressed yet. Furthermore, learning-based identi...

The present work describes the methodology for predicting the behavior of extrapolation curves obtained in radionuclide standardization by 4pb-g coincidence measurements, applied to Na, developed at the Laboratório de Metrologia Nuclear of IPEN-CNEN/SP (LMN–Nuclear Metrology Laboratory). The LMN system consists of a proportional counter (PC) in 4p geometry coupled to a single or a pair of NaI(T...

The dependence of net charge and oxygen affinity of human hemoglobin upon hemoglobin concentration was reinvestigated. In contrast to earlier reports from various laboratories, both functional properties of hemoglobin were found to be independent of hemoglobin concentration. Two findings indicate a concentration-independent net charge of carbonmonoxy hemoglobin at pH 6.6: (A) The pH value of a ...

The reconstitution of Na+/K+-ATPase from outer medulla of rabbit kidney into large unilamellar liposomes was achieved through detergent removal by dialysis of mixed micellar solutions of synthetic dioleoyl phosphatidylcholine/octyl glucoside and Na+/K+-ATPase/decyl maltoside or decenyl maltoside. Tight, transport-active liposomes were formed when the lipid and the enzyme were solubilized separa...

PTH stimulates transcellular Ca2+ absorption in renal distal convoluted tubules. The effect of PTH on membrane voltage, the ionic basis of the change in voltage, and the relations between voltage and calcium entry were determined on immortalized mouse distal convoluted tubule cells. PTH (10(-8) M) significantly increased 45Ca2+ uptake from basal levels of 2.81 +/- 0.16 to 3.88 +/- 0.19 nmol min...

The effects and modes of action of certain lipid second messengers and protein kinase C regulators, such as sphingosine, lysophosphatidylcholine (lyso-PC), and oleic acid, on Na,K-ATPase and sodium pump were examined. Inhibition of purified rat brain synaptosome Na,K-ATPase by these lipid metabolites, unlike that by ouabain, was subject to membrane dilution (i.e. inhibition being counteracted b...