Chip-scale lasers with a wide tunable range and narrow linewidth have rich applications in optical communications, sensing, light detection ranging systems. External cavity (ECLs) the advantage of tuning compared their counterparts such as distributed feedback Bragg reflector because passive filter separated from gain section can be widely tuned low-loss external extends photon lifetime. Howeve...

Gene transcription in mammalian cells is a dynamic process involving regulated assembly of transcription complexes on chromatin in which the TATA-binding protein (TBP) plays a central role. Here, we investigate the dynamic behaviour of TBP by a combination of fluorescence recovery after photobleaching (FRAP) and biochemical assays using human cell lines of different origin. The majority of nucl...

BTAF1 (formerly named TAF(II)170/TAF-172) is an essential, evolutionarily conserved member of the SNF2-like family of ATPase proteins and together with TATA-binding protein (TBP) forms the B-TFIID complex. BTAF1 has been proposed to play a key role in the dynamic regulation of TBP function in RNA polymerase II transcription. We have determined the structure of native B-TFIID purified from human...

The human RNA polymerase II transcription factor B-TFIID consists of TATA-binding protein (TBP) and the TBP-associated factor (TAF) TAF(II)170 and can rapidly redistribute over promoter DNA. Here we report the identification of human TBP-binding regions in human TAF(II)170. We have defined the TBP interaction domain of TAF(II)170 within three amino-terminal regions: residues 2 to 137, 290 to 38...

To prevent life-threatening auto-inflammation/auto-immunity in peripheral tissue such as skin, the immune system must simultaneously mount rapid protective immunity, and rapidly resolve inflammation. Dendritic Cells (DC), are highly abundant skin other tissues, mediate tolerance, confer vaccine, tumor, infectious immunity by priming T cells. Like myeloid cells, DC differentiate from bone marrow...

Abstract Objective Behavioral Survey of Traits (BeST; Andrews & Robins, 2010) is a measure used to detect behavior profiles consistent with prenatal alcohol exposure in children. This study sought create control group neurotypical individuals over the age 18, assess reliability adapted BeST Adult-Self-Report (BeST-ASR) and Adult-Other (BeST-AO). Methods A sample (n = 51, paired n 23) adults...

Cystic fibrosis (CF) is an autosomal recessive disorder and caused by variants in the Fibrosis Transmembrane Conductance Regulator (CFTR) gene. We aimed to study frequency of F508del variant, most common variant worldwide, patients with CF from Paraguay. The Paraguayan a clinical diagnosis was assessed using polymerase chain reaction followed sequencing PCR products. 43 86 (50%) were homozygous...