نتایج جستجو برای: 16s rdna pcr
تعداد نتایج: 199975 فیلتر نتایج به سال:
In 1994, the uncultured phloem-restricted bacteria of citrus huanglongbing (ex-greening) disease in Asia and Africa were characterized as 'Candidatus Liberobacter asiaticum' and 'Candidatus Liberobacter africanum', respectively. Following the rules of the International Code of Nomenclature of Bacteria, the two bacterial species have now been renamed 'Candidatus Liberibacter asiaticus' and 'Cand...
Aeromonas hydrophila is an important bacterial pathogen which causes the hemorrhagic septicemia in fishes, amphibians and humans. Genetic relationships of diverse isolates of A. hydrophila were recovered from fish and water sources. These isolates were investigated by flanked region of 16S and 23S Ribosomal DNA (rDNA) internal transcribed spacer (ITS). Here we analyzed polymorphism of PCR-ampli...
Anaplasma phagocytophilum, which causes the disease tick-borne fever (TBF), is the most important tick-borne pathogen in European animals. TBF may contribute to severe welfare challenges and economic losses in the Norwegian sheep industry. The bacterium causes a persistent infection in sheep and several other animal species. The objective of this study was to investigate whether intrauterine tr...
provides direct evidence that this arthropod is a competent vector (4). We report the first identification and characterization of an HGE-like agent in a blacklegged tick collected in a tick-endemic area of Canada (6). Sixty male and 60 female I. scapularis were collected from five white-tailed deer shot on Long Point Peninsula, Ontario, during November 1999. Live ticks were cut longitudinally ...
زمینه و هدف: پروبیوتیک ها میکروارگانیسم های غیر پاتوژن و مفیدی هستند که شناسایی آنها در محصولات لبنی سنتی نه تنها می تواند منجر به جداسازی باکتریهای پروبیوتیکی با خصوصیات ویژه شود، بلکه می تواند دیدگاه مناسبی برای تولید انبوه محصولات لبنی سنتی که به طور طبیعی حاوی باکتریهای پروبیوتیکی هستند به ما عرضه کند. مواد و روش ها: پس از نمونه برداری محصولات لبنی از مناطق مختلف شهرستان، کشت های متوالی ب...
Direct PCR enables detection of Mycoplasma pneumoniae in patients with respiratory tract infections.
The sensitivities of three methods of detection of Mycoplasma pneumoniae by a 16S rDNA PCR were compared by using a serial dilution of M. pneumoniae. These methods consisted of a PCR performed directly on cells after a proteinase K pretreatment (direct PCR), a PCR after purification of nucleic acids (DNA-PCR), and a PCR with rRNA sequences as the target after reverse transcription. The direct P...
OBJECTIVE Early and accurate diagnosis of bacterial meningitis is of critical concern. Optimum and rapid laboratory facilities are not routinely available for detecting the etiologic agents of meningitis. The objective of this study was to compare polymerase chain reaction (PCR) assay with culture for detection of bacteria in central nervous system (CNS) samples from patients suspected to have ...
ABSTRACT Diversity in host range, pathogenicity, phenotypic characteristics, repetitive extragenic palindromic polymerase chain reaction (rep-PCR) profiles, and sequence of the 16S-23S rDNA spacer region was examined among 44 Xanthomonas strains isolated from lettuce. Forty-two of the strains were divided into two groups, designated A and B. Seventy percent were Group A, and most of the remaini...
In this study we compared two polymerase chain reaction (PCR) methods using either 16S ribosomal RNA (rRNA) or 23S rRNA gene primers for the detection of different Leptospira interrogans serovars. The performance of these two methods was assessed using DNA extracted from bovine tissues previously inoculated with several bacterial suspensions. PCR was performed on the same tissues before and aft...
Increasing evidence suggests a role of the gut microbiota in colorectal carcinogenesis (CRC). To detect bacterial markers of colorectal cancer in African Americans a metabolomic analysis was performed on fecal water extracts. DNA from stool samples of adenoma and healthy subjects and from colon cancer and matched normal tissues was analyzed to determine the microbiota composition (using 16S rDN...
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