King et al. (King, K., Benkovic, S. J., and Modrich, P. (1989) 264, 11807-11815) have shown that Glu-111 is required for DNA cleavage by EcoRI endonuclease and have suggested that this residue is required for activation of the cleavage center upon specific recognition. We have substituted Gln or Asp for Glu-111 by oligonucleotide-directed mutagenesis. First and second strand cleavage rate const...

This computational study is motivated by the apparent conflict between an experiment on dissociation of H2 and D2 on Pt~111!, which suggests a rather corrugated potential energy surface ~PES! for the H2/Pt~111! system, and an experiment showing only weak nonzero-order diffraction of HD scattering from Pt~111!. In the calculations we have used density functional theory ~DFT! within the generaliz...

Introduction: Due to the cell labeling potentials of various metal-maltol complexes, the idea of developing a possible cell labeling imaging agent using single photon emission computed chromatography (SPECT) with[111In]-maltolate complex has been investigated. Methods: [111In] labeled 3-hydroxy-2-methyl-4H-pyran-4-onate ([111In]-maltolate) was prepared using f...

The simultaneous manifestation of heart disease and thyroid problems is a fairly common phenomenon. In the case of the simultaneous appearance of these complications at a advanced level of development there is the need to conduct joint endocrine surgery and a cardiac surgical operation. This is only possible in specialist units with a high level of various medical specialisations. The aim of ou...

The fibrous capsule of the tunica albuginea surrounds each testis. A break in the integrity of the tunica albuginea represents a “ruptured” testicle, which can be caused by blunt trauma. External to the testicular parenchyma and tunica albuginea is the tunica vaginalis, which envelops each testicle and fastens it to the posterior scrotal wall. The gubernaculum, or scrotal ligament, anchors each...

The alphavbeta3-integrin is expressed in angiogenic vessels in response to hypoxia and represents a potential novel target for imaging myocardial angiogenesis. This study evaluated the feasibility of noninvasively tracking hypoxia-induced alphavbeta3-integrin activation within the myocardium as a marker of angiogenesis early after myocardial infarction. Acute myocardial infarction was produced ...

8,9-Dimethoxy-5-(2-N,N-dimethylaminoethyl)-2,3-methylenedioxy-5 H-dibenzo[c,h][1,6] naphthyridin-6-one (ARC-111, topovale) is a new synthetic antitumor agent. In the current study, we show that ARC-111 is highly potent in scid mice carrying human tumor xenografts. ARC-111 was shown to be as active as camptothecin (CPT)-11 in the HCT-8 colon tumor model, and compared favorably with CPT-11 and to...

AIMS This study aims to investigate the effect of 5(S), 6(R)-7-trihydroxymethyl heptanoate (BML-111) on the Wnt5a/frizzled-5 (FZD5)/calcium/calmodulin-dependent protein kinase II (CaMKII) signaling pathway in septic mice, and to explore whether this pathway mediates the effect of BML-111 on inflammatory response in lipopolysaccharide (LPS)-induced RAW 264.7 cells. METHODS The cecal ligation a...