ECM protein enrichment and immunoblotting 100mg of frozen samples were homogenized using a polytron (Kinematica, Bohemia, NY) in 250μL of buffer C (HEPES pH7.9, MgCl2, KCl, EDTA, Sucrose, Glycerol, Sodium OrthoVanadate) of the CNMCS protein extraction kit. Lysates were then spun for 20 min. at 15,000 rpm at 4°C, the supernatant was saved (intermediate fraction 1, enriched for cytosolic proteins...

Methanol extracts from the leaves and stems of Euphorbia microphylla Linn milii were investigated for their activity against Fasciola gigantica, Taenia solium Pheritima pasthuma, respectively. Five concentrations (10–100mg/ml) each extract studied in bioassay, which involved determination time paralysis death worms. All exhibited con-siderable anthelmintic activities, order sensitivity to worms...

A 74-year-old man with a medical history of chronic renal failure and congestive heart failure underwent diagnostic colonoscopy following a positive fecal occult blood test. He was given oral antiplatelet medications including aspirin (100mg/ day) and sarpogrelate hydrochloride (100mg/day), which were stopped 5 days before colonoscopy. During the procedure, difficulty was encountered in reachin...

Urethane is a fermentation by-product and a potent animal carcinogen. Human exposure to urethane occurs through consumption of alcoholic beverages and fermented foods. Recently, CYP2E1 was identified as the primary enzyme responsible for the metabolism of C-carbonyl-labeled urethane. Subsequently, attenuation of urethaneinduced cell proliferation and genotoxicity in CYP2E1-/mice was reported. P...

Besides its approval as an adjunctive anticonvulsant, gabapentin was reported to be effective in neuropathic and inflammatory pain. Antihyperalgesic effect of gabapentin may be related to possible antiinflammatory activity. We aimed to examine the antiinflammatory effect of chronic gabapentin administration on inflammatory response in rats. Experiments were performed with the permission of the ...

OBJECTIVE Pancreatic-derived factor (PANDER, also named as FAM3B) is secreted by pancreatic α and β cells. Increasing evidence suggests that it may serve a hormonal function related to glycemic and lipid metabolism. In this study, we investigated the effects of PANDER overexpression on hepatic and adipose triglyceride metabolism in high-fat diet-fed male C57BL/6 mice. METHODS PANDER overexpre...

The effect of aged garlic extract on different biochemical parameters was studied. study included 72 Wistar albino rats, divided 12 in each group into six groups. Group I served as normal control group. To II high fat diet given control. Rats the III were administered at 200mg/kg bw. IV rats 200mg/kg.bw with diet. V atorvastatin 15mg/kg.bw VI and half dose (100mg 7.5mg/kg respectively) duration...

Background: Lipstick, a cosmetic product containing pigment, wax materials, oils and emollient that apply color, is the most widely used make-up to enhance beauty of lips. Consciously or unconsciously, lipsticks have cast spell over cultures for years its possible health implications on consistent wearer's remain subject controversy. This study evaluated commercial purchased from selected beaut...

Social isolation stress (SIS) in adolescence is accompanied by neurobehavioral disturbances and pathophysiological changes in certain regions of the CNS such as the hippocampus. In this study, we tested whether SIS impacts seizure susceptibility in postnatal male mice due to a role of hippocampal nitric oxide (NO). To do this, we used the pentylenetetrazole (PTZ) model of clonic seizures, open-...