نتایج جستجو برای: 10 selected rapd primers produced 160 bands
تعداد نتایج: 1694252 فیلتر نتایج به سال:
The random-amplified polymorphic DNA (RAPD) technique (5,7) is one of the most useful methods for species identification and studies on the genetic structure of populations of microand macro-parasites. This method generally provides numerous markers that must be cloned and labeled to be used as probes (1,4). These probes can be used to test the specificity and the polymorphism of the RAPD marke...
The arboreal species Spondias mombin L. (Anacardiaceae) is widely distributed in Brazil, where the fruits, known by the common name of cajá, are an important commercial commodity. We evaluated genetic variability among 32 cajá accessions of the Germplasm Collection of Embrapa Meio-Norte using RAPD technique. Reaction conditions for efficient RAPD amplifications were optimized in prelimina...
sex determination in pistacia as a dioecious plant is economically desirable. identification of the male and female plants has a great value for pistacia yield production. in the present study, leaf samples from four female plants from cultivars of pistacia including akbary, akbar aghaei, fandoghi, and kalleh ghochi as well as male plants were used for rapd pcr amplification. among 32 primers w...
DNA analysis by molecular techniques has significantly expanded the perspectives of the study and understanding of genetic variability in molluscs that are vectors of schistosomiasis. In the present study, the genetic variability of susceptible and resistant B. lenagophila strains to S. mansoni infection was investigated using amplification of their genomic DNA by RAPD-PCR. The products were an...
Field crop landraces are valuable genetic sources. Twenty populations of Persian clover (Trifolium resupinatum L.) collected from different areas of Iran were used in this study. DNA extractions were carried out using minipreparation method with equal amount of leaves from 30 plants of each population. DNA samples from 20 clover populations were evaluated using semi-random (ISJ) markers. Ten pr...
genetic diversity is one of principle factors to succeed in breeding programs, so its determination is vital in the using of plant genetic resources. with the aim of evaluation of genetic diversity among safflower landraces, 20 lines (14 iranian and 6 foreign lines) were studied for phenotypic and genotypic variations using agro-morphological traits and rapd markers. field study was conducted i...
Field crop landraces are valuable genetic sources. Twenty populations of Persian clover (Trifolium resupinatum L.) collected from different areas of Iran were used in this study. DNA extractions were carried out using minipreparation method with equal amount of leaves from 30 plants of each population. DNA samples from 20 clover populations were evaluated using semi-random (ISJ) markers. Ten pr...
In this study, 45 Pleurotus isolates with a wide range of geographical origins (in five provinces of Iran: Isfahan, Chaharmahal Bakhtiary, Kohkiloye boyrahmad, Lorestan and Fars) were collected. A random amplified polymorphic DNA polymerase chain reaction (RAPD-PCR) was used to assess the genetic diversity in ten populations of the Pleurotus eryngii complex. The use of ten primers allowed the p...
Finding association between molecular markers and agronomic traits provides an excellent tool for indirect selection of a trait of interest in the population. In this study, stepwise regression analysis was used to estimate associations between ISSR and RAPD markers with some agronomic traits in 12 lemon balm ecotypes. Markers or bands were considered as independent variables and traits were co...
The common split-gilled mushroom, Schizophyllum commune is found throughout the world on woody plants. This study was initiated to evaluate conditions for favorable vegetative growth and to determine molecular phylogenetic relationship in twelve different strains of S. commune. A suitable temperature for mycelial growth was obtained at 30℃. This mushroom grew well in acidic conditions and pH 5 ...
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