In this study, 112 Echinococcus granulosus isolates from different livestock of Iran were genotyped by PCR amplification of ribosomal DNA-internal transcribed spacer 1 (rDNA-ITS1) region followed by restriction fragment length polymorphism (RFLP) with the enzyme RsaI. The possibility of intra-genotype variation was also investigated using randomly amplified polymorphic DNA (RAPD) analysis. Isol...

In this study random amplified polymorphic DNA (RAPD) markers were used to infer the genetic profile of the black francolin (Francolinus francolinus, Galliformes) (N = 23) collected in five Pakistani areas (Alipur, Bait Suvai, Chakwal, Haroonabad and Rakni). A total of 269 different RAPD bands were obtained using fifteen primers. Estimates of the percentage of overall polymorphism (87.79%), Sha...

Evaluation of relationships is of prime importance in any breeding studies. In current research, genetic diversity among different rapeseed genotypes from several geographical origins including Canada, Australia, Germany, France, Denmark, Netherlands, Sweden, Iran, and Somegenotypesof unknown origin, were evaluated by RAPD and ISSR markers. All of the generated bands using 12 RAPDs and 15 ISSRs...

anopheles fluviatilis complex is known to be a vector of malaria in iran. since mosquitoes of this species cover a wide geographical range in iran, they might have evolved into different separated populations. random amplified polymorphic dna polymerase chain reaction (rapd-pcr) assay was used to differentiate geographic populations of this species. dna was extracted from individual mosquitoes ...

The screwworm, Cochliomyia hominivorax (Coquerel) (Diptera: Calliphoridae), is one of the most devastating arthropod pests of livestock in the Western Hemisphere. Early instars are very difficult to distinguish morphologically from several closely related blow fly species. Random amplified polymorphic DNA-polymerase chain reaction (RAPD-PCR) markers were developed for identifying C. hominivorax...

Molecular and phenotyping techniques were applied to study Salmonella enterica serovar Enteritidis strains both from human cases of infection and of avian origin isolated in Uruguay from 1995 to 2002. A group of 62 isolates was subjected to random amplified polymorphic DNA (RAPD) assay and analysis of antibiotic resistance patterns. Twenty-one of these strains were further characterized by phag...

A large number of micropropagated plantlets of banana, Musa acuminata var. Nanjanagudu Rasabale (NR), that were developed from axillary shoot bud explants over 10 years ago were screened for genetic variation, if any, using RAPD (Random Amplified Polymorphic DNA) and ISSR (Inter-Simple Sequence Repeats) markers. Of the 4000 in vitro plantlets, 11 were used for screening that involved shoot cult...

Randomly amplified polymorphic DNA (RAPD) markers were used to study the genetic diversity and discriminate among 17 unknown genotypes (considered potentially salt tolerant) and 16 known olive cultivars. Fifteen decamer primers which produced 38 reproducible polymorphic bands in the genotypes were selected for analysis. The RAPD markers resulted in 93 distinct banding patterns. Based on either ...

Due to the limited number of molecular studies focused on European gene pool investigation, it is necessary to perform plant material recognition. Eighteen accessions of three Miscanthus species, namely, M. × giganteus, M. sinensis, M. sacchariflorus were evaluated with the use of molecular marker systems such as: inter simple sequence repeats (ISSRs), random amplified polymorphic DNA (RAPD), a...