The Rockefeller University Press $30.00 J. Cell Biol. Vol. 208 No. 2 239–248 www.jcb.org/cgi/doi/10.1083/jcb.201410042 JCB 239 Correspondence to Stéphane Vincent: [email protected] Abbreviations used in this paper: ANOVA, analysis of variance; Brk, Brinker; DC, dorsal closure; DPP, Decapentaplegic; FFL, feed-forward loop; Jar, Jaguar; LE, leading edge; tkv, thick veins; UAS, upstre...

During mitosis, the duplicated genome undergoes a dramatic structural reorganization, resulting in condensed, resolved chromosomes that can be segregated by the spindle during anaphase. Core histones H2A, H2B, H3, and H4 provide the first level of genome compaction, assembling into stable octameric units around which DNA is encircled to form nucleosomes. Linker histones bind nucleosomes and the...

In multicellular organisms, cellular differences in gene activity are a prerequisite for differentiation and establishment of cell types. In order to study transcriptome profiles, specific cell types have to be isolated from a given tissue or even the whole organism. However, wholetranscriptome analysis of early embryos in flowering plants has been hampered by their size and inaccessibility. He...

Skeletal patterning in the sea urchin embryo requires a conversation between the skeletogenic primary mesenchyme cells (PMCs) and the overlyingpattern-dictatingectoderm;however, our understandingof the molecular basis for this process remains incomplete. Here, we show that TGF-β-receptor signaling is required during gastrulation to pattern the anterior skeleton. To block TGF-β signaling, we use...

Correspondence to Takanobu Otomo: [email protected]; or Thomas Braulke: [email protected] T. Otomo’s present address is Department of Genetics, Osaka University Graduate School of Medicine, 5650871 Suita, Osaka, Japan. Abbreviations used in this paper: Fuc, -fucosidase; Man, -mannosidase; Gal, -galactosidase; Hex, -hexosaminidase; CFSE, carboxyfluorescein succinimidyl ester; Cts,...

The identification and characterization of the cellular and molecular pathways involved in the differentiation andmorphogenesis of specific cell types of the developing heart are crucial to understanding the process of cardiac development and the pathology associated with human congenital heart disease. Here, we show that the cardiac transcription factorCASTOR(CASZ1)directly interactswithcongen...

High flavonoid consumption can improve vascular health. Exploring flavonoid–metabolome relationships in population-based settings is challenging, as: (i) there are numerous confounders of the flavonoid–metabolome relationship; and (ii) the set of dependent metabolite variables are inter-related, highly variable and multidimensional. The Metabolite Fingerprint Score has been developed as a means...

In honey bees (Apismellifera), the epigeneticmark of DNAmethylation is central to the developmental regulation of caste differentiation, but may also be involved in additional biological functions. In this study, we examine the whole genome methylation profiles of three stages of the haploid honey bee genome: unfertilised eggs, the adult drones that develop from these eggs and the sperm produce...

The directed movement of cells is a fundamental aspect of tissue morphogenesis. Cells migrating in vivo often must invade tissue barriers and ultimately form new cellular contacts in their target tissues. Furthermore, in many epithelial tissues proliferation occurs via a population of basal stem cells that divide below the apical surface and move apically to join the epithelium (Rock and Hogan,...

Pitt-Hopkins syndrome (PTHS) is caused by haploinsufficiency of Transcription factor 4 (TCF4), one of the three human class I basic helix-loop-helix transcription factors called E-proteins. Drosophila has a single E-protein, Daughterless (Da), homologous to all three mammalian counterparts. Here we show that human TCF4 can rescue Da deficiency during fruit fly nervous system development. Overex...