Gamma radiation effectively controls Listeria monocytogenes in uncooked and in ready-to-eat foods. This study was conducted to determine if gamma radiation could be used to control L. monocytogenes in ground pork. Ground pork was contaminated with L. monocytogenes, kept at refrigeration (4 degrees C), chilling (0 degrees C), and freezing (-18 degrees C) temperatures overnight, exposed to gamma ...

the purpose of this preliminary study was to determine the prevalence of raw milk contamination with listeria monocytogenes. in this study, 100 bulk tank milk samples were collected randomly and delivered to pegah pasteurization factory in mashhad.for isolation and identification of l. monocytogenes, the samples were first enriched using cold enrichment method in listeriaenrichment broth, follo...

Listeria monocytogenes is an intracellular bacterium that causes systemic infections after traversing the intestinal mucosa. Clearance of infection and long term protective immunity are mediated by L. monocytogenes-specific CD8 T lymphocytes. In this report, we characterize the murine CD8 T cell response in the lamina propria and intestinal epithelium after enteric L. monocytogenes infection. W...

The capability of an assay to detect Listeria monocytogenes from artificially inoculated fresh-cut produce such as cantaloupe and mixed salad was demonstrated. An oligonucleotide probe that becomes fluorescent upon hybridization to the target DNA (Molecular Beacon, MB) was used in a real-time polymerase chain reaction (PCR) assay. As few as 4 to 7 colony-forming units (CFU) of L. monocytogenes ...

Listeria monocytogenes is an important foodborne pathogen. The aims of this study were to determine genetic relatedness of L. monocytogenes isolated from ready-to-eat (RTE) foods in Malaysia. L. monocytogenes isolates from RTE foods were characterized by multiplex-PCR serotyping, REP-PCR, BOX-PCR, RAPD, PFGE, virulotyping and antibiotyping. Of the 32 L. monocytogenes isolates analyzed, 21 (65.6...

Human-monocyte-derived dendritic cells (MoDC) are very efficient in the uptake of Listeria monocytogenes, a gram-positive bacterium which is an important pathogen in humans and animals causing systemic infections with symptoms such as septicemia and meningitis. In this work, we analyzed the influence of blood plasma on the internalization of L. monocytogenes into human MoDC and compared the upt...

The objectives of this study were to examine and develop a model to describe the survival of Listeria monocytogenes in salmon as affected by salt, smoke compound (phenol), and smoking process temperature. Cooked minced salmon containing selected levels of salt (0%, 2%, 4%, and 6%) and smoke compound (0, 5, 10, and 15 ppm phenol) were inoculated with a 6-strain mixture of L. monocytogenes to an ...

This study evaluated the effect of EDTA and lysozyme on the antibacterial activities of activated ovotransferrin against 5 strains of Listeria monocytogenes. First, a disc test was performed to screen the concentrations of EDTA or lysozyme that showed antibacterial activities in ovotransferrin (O) or ovotransferrin in 100 mM NaHCO3 (OS) solution. Turbidity and viability tests were conducted usi...

Listeria monocytogenes is well known for having the ability to cross the placental barrier, leading to fetal infections and abortion. However, the mechanisms leading to infectious abortion are poorly understood. In this study, we demonstrate that interferon γ-induced GTPase (IGTP) contributes to the invasion of L. monocytogenes into trophoblast giant (TG) cells, which are placental immune cells...