Studies on the β-galactosidase production were carried out with a bacterial strain isolated from the soil sample near dairy processing plant. The production of extracellular β-galactosidase by Bacillus sp. was optimized in a submerged fermentation. The effect of incubation period, temperature and pH of the medium were optimized. The production of the enzyme was found to be high at the 48th hour...

The gal3 mutation of Saccharomyces, which is associated with an impairment in the utilization of galactose, has been shown to be pleiotropic, causing similar impairments in the utilization of melibiose and maltose. Milibiose utilization and alpha-galactosidase production are directly controlled by the galactose regulatory elements i, c, and GAL4. The fermentation of maltose and the induction of...

This Application Note describes the development of an immunoprecipitation method in combination with microfluidic on-chip analysis on the Agilent 2100 Bioanalyzer for sensitive, selective and quantitative detection of endogenous β-galactosidase in crude E. coli lysates. The effects of antibody selection and the importance of preclearing steps for sensitive and specific detection of the target p...

We describe a new chromogenic agar medium, ABC medium (alphabeta-chromogenic medium), which includes two substrates, 3, 4-cyclohexenoesculetin-beta-D-galactoside and 5-bromo-4-chloro-3-indolyl-alpha-D-galactopyranoside, to facilitate the selective isolation of Salmonella spp. This medium exploits the fact that Salmonella spp. may be distinguished from other members of the family Enterobacteriac...

Specific activities of seven acid glycosidases: beta-hexosaminidase, alpha- and beta-galactosidase, alpha- and beta-mannosidase, alpha-glucosidase and alpha-fucosidase were determined in various parts of the domestic hen oviduct (infundibulum, isthmus, shell gland and vagina). The activity of most enzymes was the highest in the isthmus. Multiple forms of all acid glycosidases from the isthmus w...