In this work, peptides were extracted from Silver carp (SC) scales via protease hydrolysis and separated using two membranes (M1 M2). The results revealed that the water: SC scale ratios of 50.6 mL/g, alkaline 1 (AP1) dose 2313.6 U/mL, pH 8.14 optimal conditions, peptide yield reached 88.77 ± 0.32%. conditions separation clarified: operating pressure M1 (M2) was 0.25 (0.4) MPa, liquid temperatu...

Cystatins are proteins, which inhibit cysteine proteases, such as papain. In this study, the 336-bp cystatin C gene (family II, HmCysC) of silver carp (Hypophthalmichthys molitrix) was cloned and expressed in Escherichia coli BL21 (DE3). HmCysC encodes mature peptide (HmCystatin C), with 111 amino acids. A typical QXXXG motif found HmCystatin it formed a cluster Cyprinus carpio Danio rerio phyl...

The effectiveness of chemical compounds for marking hard tissues in juvenile silver carp (Hypophthalmichthys molitrix) is not well known. We analyzed the use alizarin complexone (ALC) as a fluorescent marker to mark various structures carp. Experimental fish (~2 months old) were randomly assigned either control or groups, which immersed 0 100 mg/L ALC solutions, respectively, 2 days. otoliths, ...