Alterations in cellular metabolism are among the most consistent hallmarks of cancer. Herein we have investigated the relationship between increased aerobic lactate production and mitochondrial physiology in tumor cells. To diminish the ability of malignant cells to metabolize pyruvate to lactate, lactate dehydrogenase A (LDH-A) levels were knocked down by means of LDH-A short hairpin RNAs. Red...

Human isoenzyme LDH-X (lactate dehydrogenase isoenzyme X) was isolated from seminal fluid of frozen semen samples by affinity chromatography by using oxamate-Sepharose and AMP-Sepharose. In the presence of 1.6 mM-NAD+, isoenzyme LDH-X does not bind to AMP-Sepharose, whereas the other lactate dehydrogenase isoenzymes do. This is the crucial point in the isolation of isoenzyme LDH-X from the othe...

Although the levels of LDH have been estimated in the aqueous humour of animals (Kuhlman, and Kaufman, I960), information regarding LDH levels in human aqueous humour appears to be scanty. It is known that malignant tumours at different sites in the body are associated with an increased LDH activity in the corresponding body fluid (Wroblewski, 1957). The purpose of the present study is to deter...

We report the adsorption of dextranase on a Mg/Fe-layered double hydroxide (Mg/Fe-LDH). We focused the effects of different buffers, pH, and amino acids. The Mg/Fe-LDH was synthesized, and adsorption experiments were performed to investigate the effects. The maximum adsorption occurred in pH 7.0 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) buffer, and the maximum dextranase adsorp...

A layered double hydroxide (LDH) film was formed in situ on aluminum alloy 2024 through a urea hydrolysis method, and a decavanadate-intercalated LDH (LDH-V) film fabricated through the dip coating method. The microstructural and morphological characteristics were investigated by scanning electron microscopy (SEM). The corrosion-resistant performance was analyzed by electrochemical impedance sp...

Serum total lactate dehydrogenase (LDH) was estimated serially for up to 15 days in 110 patients with head injuries who had no extraneural injury. Increase in LDH activity generally indicated the degree of parenchymal brain damage. The prognostic value of LDH was established by correlating its activity with clinical criteria such as duration of unconsciousness and post-traumatic amnesia, type o...

Abstract Coconut-fiber biochar (CCFB) pyrolyzed at different pyrolysis temperatures (400°C, 500°C, and 600°C) that immobilized Zn/Al layered double hydroxides (Zn/Al LDH-CCFB) composite adsorbent was prepared via a simple co-precipitation technique. The adsorbents were used to reduce the high concentration of Ni(II) metal ions from aqueous solution. morphological analysis investigated scanning ...

The enzyme activity and the electrophoretic characteristics of lactic dehydrogenase (LDH) in the plasma and different tissues from the mice with radiation-induced lymphoid leukaemia were examined and compared with those obtained from the normal mice. The LDH activity was ab­ normally increased in the leukaemic plasma. A considerable elevation of the enzyme level was also found both in the leuka...

Four pyridine nucleotide-linked dehydrogenases, glucose-6phosphate dehydrogenase (G6PDH),3 isocitric dehydrogenase (ICDH), lactic dehydrogenase (LDH), and malic dehydrogenase (MDH), and their isozymes have been studied in the normal virgin rat breast, in the breast of tumor-bearing rats, in the breast cancers themselves, and in these tissues following ovariectomy. Enzyme activity expressed per ...