Loop-mediated isothermal amplification (LAMP) constitutes a potentially valuable diagnostic tool for rapid diagnosis of contagious diseases. In this study, we developed a novel LAMP method (seM-LAMP) to detect the seM gene of Streptococcus equi subsp. equi (S. equi), the causative agent of strangles in equids. The seM-LAMP successfully amplified the target sequence of the seM gene at 63°C withi...

Background and aims Pornography use has become increasingly common. Studies have shown that individuals who use sexually explicit materials (SEMs) report negative effects (Schneider, 2000b). However, Bridges (2008b) found that couples who use SEM together have higher relationship satisfaction than those who use SEM independently. A further investigation into various types of SEM use in relation...

In t ramembranous charge movement was measured in cut twitch fibers mounted in a double Vaseline-gap chamber with ei ther a tetraethylammonium chloride (TEA.CI) o r a TEA2.SO 4 solution (13-14~ in the central pool. Charge vs. voltage data were fitted by a single two-state Boltzmann distr ibution function. The average values of V (the voltage at which steady-state charge is equally distr ibuted ...

Since the Scanning Electron Microscope (SEM) was first commercialized about 40 years ago, the SEM has shown a remarkable progress. Now, many types of SEMs are being used, and their performance and functions are greatly different from each other. To utilize these different SEMs, it is essential to recognize their features, as well as to understand the reasons for the contrast of SEM images. Thus...

We identify ghost trajectories of symplectic-energy-momentum (SEM) integration and show that the ghost trajectories are not time reversible. We explain how SEM integration can be regularized, in a SEM preserving manner, so that it is time reversible. We describe an algorithm for implementing the regularized SEM integrator. Simulation results for the pendulum are given. Coordinate invariance of ...

introduction: this study was carried out to investigate calcific tissue formation against mineral trioxide aggregate (mta) after pulp capping. materials and methods: the pulps of six teeth from four dogs were exposed and capped with mta. after extraction each tooth was sectioned into halves. each half was then further sectioned in the mesiodistal or buccolingual direction. calcified tissue of t...

morphological and histochemical study of the abomasal epithelium in camel (camelus dromedarius) wascarried out by light and scanning electron microscopes. the lining of the abomasum was divided into fourregions, i.e. cardiac, pseudocardiac, fundic and pyloric. our investigation revealed that the cardiac andpseudocardiac regions occupy a wide part of the abomasum in camel and it reaches approxim...

the study reports some preliminary investigations on the template free synthesis of a scantly investigated polyaniline (pani) derivative-phenylenediamine (pda) by template free method in the presence of oxalic acid (oa) (dopant), using ammonium persulfate as oxidant. polymerization of phenylenediamine (pda) was confirmed by the ft-ir as well as uv–visible studies. the morphology of phenylenedia...