We describe an improved gel-based method with an additive Zn(2+)-cyclen complex (cyclen, 1,4,7,10-tetraazacyclododecane), Zn(2+)-cyclen-PAGE, for mutation detection in DNA fragments by PCR that contain more than 65% Ade/Thy bases and fewer than 100base pairs (bp). Existing techniques have a problem in analyzing such short Ade/Thy-rich fragments because the duplexes are disrupted and are not det...

Let Σ be a smooth projective complex curve and g a simple Lie algebra of type ADE with associated adjoint group G. For a fixed pair (Σ, g), we construct a family of quasiprojective Calabi-Yau threefolds parameterized by the base of the Hitchin integrable system associated to (Σ, g). Our main result establishes an isomorphism between the Calabi-Yau integrable system, whose fibers are the interme...

nature of Hex+Cxcr4+ (H+C+) ES cell derived ADE. Global expression analysis of ES cell derived ADE lead to the identification of an H+C+ gene expression cluster that contains well know anterior endoderm markers alongside a novel set of new sequence tags. Based on multiple pairwise comparisons between the H+C+ fraction and both differentiated and undifferentiated ES cell fractions, 66 genes were...

Extracellular adenosine (ADE) interacts with cells by two pathways: by activating cell surface receptors at nanomolar/micromolar concentrations; and by interfering with the homeostasis of the intracellular nucleotide pool at millimolar concentrations. Ade shows both cytotoxic and cytoprotective effects; however, the underlying mechanisms remain unclear. In the present study, the effects of aden...

Vector-based information retrieval methods such as the vector space model (VSM), latent semantic indexing (LSI), and the generalized vector space model (GVSM) represent both queries and documents by high-dimensional vectors learned from analyzing a training corpus of text. VSM scales well to large collections, but cannot represent term–term correlations, which prevents it from being used in tra...

nature of Hex+Cxcr4+ (H+C+) ES cell derived ADE. Global expression analysis of ES cell derived ADE lead to the identification of an H+C+ gene expression cluster that contains well know anterior endoderm markers alongside a novel set of new sequence tags. Based on multiple pairwise comparisons between the H+C+ fraction and both differentiated and undifferentiated ES cell fractions, 66 genes were...

Ross River virus (RRV) is the aetiological agent of epidemic polyarthritis (EPA) a predominantly rheumatic disease afflicting up to 5000 Australians annually. We show here for the first time that macrophages can be productively infected by RRV. Subneutralizing titres of anti-RRV IgG (but not IgM) also showed classical antibody-dependent enhancement (ADE) of RRV infection in macrophage and monoc...

A great body of previous research shows that personalization is a highly desirable method for online content delivery, based on the offline model of one to one personal interactions. Many different engines have been proposed to support such personalization on the Web. They all however have some weaknesses, which may have been partially responsible for the slow uptake of these promising techniqu...

Thirty-two pregnant patients at term undergoing elective Caesarean section were ventilated with a non-co-axial ADE anaesthetic system (E mode) supplied with fresh gas flows (FGF) of either 70 or 100 ml kg-1 min-1, on a random basis. Ventilation with an FGF of 70 ml kg-1 min-1 produced mean PaCO2 and PE' CO2 values of 6.48 +/- 1.15 kPa and 6.41 +/- 0.76 kPa, respectively. Patients were thus hype...

Dengue virus co-circulates as four serotypes, and sequential infections with more than one serotype are common. One hypothesis for the increased severity seen in secondary infections is antibody-dependent enhancement (ADE) leading to increased replication in Fc receptor-bearing cells. In this study, we have generated a panel of human monoclonal antibodies to dengue virus. Antibodies to the stru...