نتایج جستجو برای: مد tem

تعداد نتایج: 47549  

Journal: :The Journal of Experimental Medicine 1992
W A Muller S A Weigl

We describe a quantitative assay of transendothelial migration (TEM) that allows us to selectively study the interaction of monocytes with confluent human endothelial cell (HEC) monolayers. The HEC are grown on hydrated collagen gels; the monocytes need not be purified. 100% of monocytes transmigrated the monolayer within 1 h at 37 degrees C and accumulated in the subendothelial collagen; TEM o...

2003
William A. Muller Susan A. Weigl

We describe a quantitative assay of transendothelial migration (TEM) that allows us to selectively study the interaction of monocytes with confluent human endothelial cell (HEC) monolayers. The HEC are grown on hydrated collagen gels; the monocytes need not be purified. 100% of monocytes transmigrated the monolayer within 1 h at 37~ and accumulated in the subendothelial collagen; TEM oflymphocy...

Journal: :The Journal of antimicrobial chemotherapy 2007
Mariagrazia Perilli Giuseppe Celenza Marianna Fiore Bernardetta Segatore Cristina Pellegrini Francesco Luzzaro Gian Maria Rossolini Gianfranco Amicosante

OBJECTIVES Kinetic characterization of TEM-134, a new TEM-type extended-spectrum beta-lactamase variant isolated from Citrobacter koseri during an Italian nationwide survey. TEM-134 is a natural derivative of TEM-2 with the following substitutions: E104K, R164H and G238S. METHODS Recombinant TEM-134 was purified from Escherichia coli HB101 (pMGP-134) by three chromatographic steps (cation-exc...

Journal: :Nano letters 2015
Jungwon Park Hyesung Park Peter Ercius Adrian F Pegoraro Chen Xu Jin Woong Kim Sang Hoon Han David A Weitz

Recent development of liquid phase transmission electron microscopy (TEM) enables the study of specimens in wet ambient conditions within a liquid cell; however, direct structural observation of biological samples in their native solution using TEM is challenging since low-mass biomaterials embedded in a thick liquid layer of the host cell demonstrate low contrast. Furthermore, the integrity of...

2011
Andrea Fasoli Alan Colli Faustino Martelli Simone Pisana Ping Heng Tan Andrea C. Ferrari

The crystal phase of Au-NWs is assessed by means of high-resolution transmission electron microscopy (HR-TEM). NWs are dispersed on a transmission electron microscopy (TEM) grid and imaged with a JEOL 4000EX-II TEM. Figure S-2(a) shows a HR-TEM lattice imaging of a representative Au-NW. The measured interplanar spacing (3.74 Å) is consistent with the (110) planes of a CdSe crystal with the wurt...

Journal: :Journal of microscopy 2006
M Marko C Hsieh W Moberlychan C A Mannella J Frank

The feasibility of using a focused ion beam (FIB) for the purpose of thinning vitreously frozen biological specimens for transmission electron microscopy (TEM) was explored. A concern was whether heat transfer beyond the direct ion interaction layer might devitrify the ice. To test this possibility, we milled vitreously frozen water on a standard TEM grid with a 30-keV Ga(+) beam, and cryo-tran...

Journal: :Journal of immunology 2003
Karkada Mohan Devanand Pinto Thomas B Issekutz

During inflammation, T lymphocytes migrate out of the blood across the vascular endothelium in a multistep process. The receptors mediating T cell adhesion to endothelium are well characterized; however, the molecules involved in T cell transendothelial migration (TEM) subsequent to lymphocyte adhesion to the endothelium are less clear. To identify receptors mediating TEM, mAbs were produced ag...

Journal: :Antimicrobial agents and chemotherapy 1998
Y Yang N Bhachech P A Bradford B D Jett D F Sahm K Bush

Ceftazidime-resistant Escherichia coli and Klebsiella pneumoniae (49 and 102 isolates, respectively) were collected from Barnes-Jewish Hospital, St. Louis, Mo., from 1992 to 1996. They were uniformly resistant to ceftazidime, generally resistant to aztreonam, and variably susceptible to cefotaxime. Four representative E. coli strains and 15 Klebsiella strains were examined. From one to four bet...

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