Site-specific photocleavage of hen egg lysozyme and bovine serum albumin (BSA) by N-(l-phenylalanine)-4-(1-pyrene)butyramide (Py-Phe) is reported. Py-Phe binds to lysozyme and BSA with binding constants 2.2 +/- 0.3 x 10(5) M-1 and 6.5 +/- 0.4 x 10(7) M-1, respectively. Photocleavage of lysozyme and BSA was achieved with high specificity when a mixture of protein, Py-Phe, and an electron accepto...

We have recently demonstrated that current heavy smokers with >20 pack-year (PY) smoking history (median, 48.8, IQR, 31.6–57.0 PY) and non-heavy (<20 PY; median, 11.4; 7.3–14.4 about 40- 10-times higher risk of lung cancer, respectively (JAMA-Oncol. 2022; 8:1428–1437). In the study, we examined magnitude graded increase associated PY among smokers. Of 2505 community-dwelling older (mean age, 73...

With >90 known deposits containing original reserves of >2400 Mt sulfide ore, the Iberian Pyrite Belt (IPB) is largest volcanogenic massive (VMS) province on Earth. In these evolving mineral systems, texturally different pyrite exhibits characteristic mineralogy and trace element fingerprints. (Py-1), which well preserved in polymetallic ores that crystallized at earliest stage VMS deposit form...

New N4O2-donor acyclic chelators 2-[[2-[2-(3,5-dimethylpyrazol-1-yl)ethyl-[2-[(2-hydroxyphenyl)methylamino]ethyl]amino]ethylamino]methyl]phenol (H2L(pz*,NH)) and 2-[[2-[2-[(2-hydroxyphenyl)methylamino]ethyl-(2-pyridylmethyl)amino]ethylamino]methyl]phenol (H2L(py,NH)) were obtained upon introduction of pyridyl or pyrazolyl coordinating units at the central nitrogen atom of diethylenetriamine (di...

• Symbols denoting random variables are capitalized but symbols denoting their realization are not. • Bold face is used for vectors. • Blackboard bold or non-italic face are used for sets, calligraphic face for system of sets. • We use x̄ as a shorthand for the difference 1 − x for x ∈ [0, 1]. • Z is the set of all integers and Z+ = {z ∈ Z : z ≥ 0}. • 2 is the set {0, 1}. • [n] denotes the set {...

Our objective was to study the incidence of sperm-tail phosphotyrosine immunoreactivity in normozoospermic and asthenozoospermic human sperm samples, its association with sperm motion parameters, particularly hyperactivated motility, and its potential involvement in the pathogenesis of asthenozoospermia. The work was conducted as a prospective experimental study in the Sperm Biology and Androlo...

We describe a novel approach to study tyrosine-phosphorylated (PY) integrins in cells transformed by virally encoded tyrosine kinases. We have synthesized a peptide (PY beta 1 peptide) that represents a portion of the cytoplasmic domain of the beta 1 integrin subunit and is phosphorylated on the tyrosine residue known to be the target of oncogenic tyrosine kinases. Antibodies prepared against t...

ÐPhotocleavage of proteins by a series of organic probe molecules is examined as a function of probe structure. For example, pyrenyl peptides Py-Gly-X (XˆTrp, Tyr, Phe, and His, and Pyˆ4(1-pyrenyl)butyroyl)) are prepared, and their protein binding/photocleavage properties have been examined. The binding constants with bovine serum albumin (BSA) are in the range of 10 to 10 and binding of the pr...

Carbohydrate-active enzymes (CAZymes) are involved in the metabolism of glycoconjugates, oligosaccharides, and polysaccharides and, in the case of plant pathogens, in the degradation of the host cell wall and storage compounds. We performed an in silico analysis of CAZymes predicted from the genomes of seven Pythium species (Py. aphanidermatum, Py. arrhenomanes, Py. irregulare, Py. iwayamai, Py...