there is a great interest in developing rapid analytical methods and provide appropriate parametersfor quantitative analysis of compounds derived from refractory pollutants. such methods are important forroutine quality control analysis of an effluent before being discharged into the environment. therefore, a rapid methodology for identification and quantification of some aliphatic acids is pro...

A simple, precise, specific ,robust and rapid HPLC assay for the of measurement meloxicam in pharmaceutical formulations was developed and validated at Department of Pharmacy and Central Quality Assurance Laboratory, LCWU, Lahore. The solution of meloxicam standard was prepared in HPLC grade acetonitrile (5 μg.mL) and ultra sonicated for 15 minutes. One mL sample mixed well with one mL of HPLC ...

A combination of hydrophobic chromatography on phenyl-Sepharose and reversed phase HPLC was used to purify individual tRNAs with high specific activity. The efficiency of chromatographic separation was enhanced by biochemical manipulations of the tRNA molecule, such as aminoacylation, formylation of the aminoacyl moiety and enzymatic deacylation. Optimal combinations are presented for three dif...

We present a candidate Reference Method for determining the concentration of serum creatinine. The method is based on HPLC combined with enzymatic determination. Creatinine plus 14[C]creatinine is extracted by cation-exchange chromatography, subjected to reversed-phase HPLC, and finally quantified enzymatically. Enzymatic measurement ensures no interference from co-eluting compounds, which has ...

High-performance liquid chromatography-solid phase extraction-NMR spectroscopy (HPLC-SPE-NMR) has recently become commercially available and has been evaluated with regard to its applicability in a pharmaceutical environment. The addition of an automated SPE unit to an HPLC-NMR system for peak trapping results in an improved NMR signal-to-noise ratio (S/N) and also has other practical advantage...

Kajian ini dijalankan untuk menilai kemampuan kaedah penentuan jumlah homosisteina dalam plasma dengan menggunakan kromatografi cecair berprestasi tinggi jenis penukar ion (HPLC-IEC) dan membandingkan kit komersil yang fasa berbalik (HPLC-RPC). Validasi bagi HPLC-IEC telah dilakukan mengikut panduan dikeluarkan oleh Jabatan Standard Malaysia. Sebanyak 57 sampel daripada pesakit dihantar diuji s...

High-performance liquid chromatography has replaced several spectroscopic methods and gas in the quantitative qualitative analysis of medications pre-sale procedure testing done past ten years. This is as part marketing drugs their control. During early stages its application, high-performance (HPLC) was expected to be a that would supplement chromatography; nevertheless, today, HPLC almost ent...

The aim of this study was to developed HPLC simultaneous analysis method for the determination Caffeoylquinic acid isolated from leaf Eribotrya japonica Lindl. extract. phenolic compounds in E. leaves were and identified by HPLC, NMR, Mass etc. quantitative carried out HPLC-DAD using C18 column with gradient elution water acetonitrile at 280 nM 60 min. successfully validated specificity, LOD (l...

In the present study, an effective method of preparative high-performance liquid chromatography (Prep-HPLC) was established to purify two taxanes in Taxus cuspidata. During experimental operation, effects flow rate, injection volume, and column temperature on purity 10-deacetyltaxol (10-DAT) paclitaxel (PTX) were investigated, optimized conditions as follows: rate 10 mL/min, volume 0.5 mL, 30 °...

The esterification of galacturonic acid with methanol and/or acetic is important for the structural analysis pectin. Although several methods have been reported determining degree methylesterification (DM) and acetylation (DAc), present study compares optimizes three (titration, FT-IR HPLC) using commercial citrus pectin (CP). Our results showed that DM CP was 47.0%, 47.6% 48.0% as determined b...