The structural gene for NAD+-dependent 3-hydroxyisobutyrate dehydrogenase (EC 1.1.1.31) from Pseudomonas putida E23 was cloned in Escherichia coli cells to obtain a large amount of the enzyme and its nucleotides were sequenced to study its structural relationship with other proteins. The gene encoded a polypeptide containing 295 amino acid residues and was in a cluster with the gene for methylm...

0191 January 2008 | Volume 6 | Issue 1 | e5 | e23 Correction: Quantitative Characteristics of Gene Regulation by Small RNA Erel Levine, Zhongge Zhang, Thomas Kuhlman, Terence Hwa Correction for: Levine E, Zhang Z, Kuhlman T, Hwa T (2007) Quantitative characteristics of gene regulation by small RNA. PLoS Biol 5(9): e229. doi:10.1371/journal.pbio.0050229 The authors would like to add the second a...

a r t i c l e i n f o JEL classification: C53 G17 Keywords: GARCH Higher conditional moments Approximate predictive distributions Value-at-Risk S&P 500 Treasury bill rate Euro–US dollar exchange rate It is widely accepted that some of the most accurate Value-at-Risk (VaR) estimates are based on an appropriately specified GARCH process. But when the forecast horizon is greater than the frequency...

The O-Ring theory is used to analyze the emergence of ̄rms organized as partnerships. The owner-managers of such entrepreneurial ̄rms bene ̄t from ability matching within their production teams. However, they must bear the project risk. Risk-aversion then induces a second-best solution. Integrated ̄rms managed on behalf of riskneutral residual-claimants face information and/or enforcement problem...

The goal of this study was to characterize a series of antiHer2/neu immunotoxin constructs to identify how different antibodies and linker choices affect the specificity and cytotoxicity of these proteins. We constructed a series of immunotoxins containing either the human single-chain antibody (scFv) C6.5 or the murine scFv e23 fused to the highly toxic recombinant gelonin (rGel) molecule. Bas...

Using the enteropathogenic Escherichia coli (EPEC) genome sequence, we found that EPEC E2348/69 has an lpfABCDE gene cluster homologous (about 60% identical at the protein level) to the Salmonella long polar fimbria (LPF) operon. To determine whether this operon is essential for adherence, the lpfABCD(E2)(3) genes were deleted from EPEC strain E2348/69 by allelic exchange. Analysis of the resul...

Bu çalışmada, sol-jel spin kaplama yöntemi kullanılarak mikroskop cam altlıklar üzerine TiO2, TiO2/CoPc ve CoPc filmleri hazırlanmış katkı konsantrasyonunun filmlerin optik yapısal özellikleri etkisi araştırılmıştır. İnce geçirgenliğini ölçmek için 300-1100 nm dalgaboyu aralığında bir spektrofotometre kullanılmıştır. Uv-Vis çalışmaları, B Q bandı absorpsiyon bölgelerine karşılık gelen aralıklar...