The present study was undertaken to delineate single nucleotide polymorphisms within exon 2 and its flanking regions of bubaline leptin gene using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. A total of 118 buffaloes from seven different breeds were studied, revealing three distinct SSCP variants, viz., A, B and C, with a frequency of 0.703, 0.195 and 0...

Dideoxy fingerprinting (ddF) was used as a tool to search for a generic set of conditions with sufficient power to detect virtually all mutations. For each condition tested, a very large sample of mutation-containing, single-stranded segments (about 1500) were analyzed with ddF. Correlation coefficients identified pairs of conditions in which single-strand conformation polymorphism (SSCP) mobil...

BACKGROUND The level of intra-species genetic variation in Entamoeba histolytica, Entamoeba dispar and Entamoeba moshkovskii populations in a localized geographic area, like Puducherry, India, remains unknown. METHODS In the present study the existence of genetic variation in the nested multiplex polymerase chain reaction (NM-PCR) amplified region of the 16S-like ribosomal RNA genes of E. his...

Combining the use of PCR and single-strand conformation polymorphisms (SSCP), nine sequences from the cucumber genome were successfully identified and cloned that encoded two well-conserved asparagine-proline-alanine (NPA) domain homologues to aquaporin genes. The sensitivity and detection efficiency of SSCP and restriction enzyme analysis for detecting DNA sequence variation were evaluated usi...

OBJECTIVE To compare genotypes of Candida albicans strains causing different conditions of vulvovaginal candidiasis (VVC) in Chinese women. METHODS C albicans strains were isolated from the vaginas of patients with different conditions of VVC. The genotypes of the strains were investigated based on single-strand conformation polymorphisms (SSCP) of the PCR amplified microsatellite locus CAI. ...

We investigated mutations in the genes katG, inhA (regulatory and structural regions), and kasA and the oxyR-ahpC intergenic region of 97 isoniazid (INH)-resistant and 60 INH-susceptible Mycobacterium tuberculosis isolates obtained in two states in Brazil: São Paulo and Paraná. PCR-single-strand conformational polymorphism (PCR-SSCP) was evaluated for screening mutations in regions of prevalenc...

The purpose of this study was to assess and explain the relationship between molecular changes occurring in squamous cell laryngeal carcinoma. Furthermore, we were revealed the role of FHIT gene response in the development and progression of squamous cell laryngeal carcinoma, and genes based exons 7,8 and 9 in tumour stage and histopathologic grade. In this study was used 50 blood samples for c...