The cell surface areas of 3T3 and SV40-3T3 cells both for suspended and for flat cells growing on the culture substrate have been determined in dependence on growth density. The surface areas of flat 3T3 and SV40-3T3 cells turn out to be independent of growth density over a wide range. For 3T3 cells the surface area of flat cells compares well with that determined by Collard and Temmink for sus...

Proteins related to simian virus 40 (SV40) T antigen uere isolated from cells infected with adenovirus 2/SV40 hybrids Ad2+D2 and Ad2+ND1 dp2 as well as from a line of human cells (SV80) transformed by SV40. The 96,000- and 107,000-dalton proteins of SV80 and Ad2+D2, after injection into the cytoplasm of cultured cells, rapidly accumulate in the nuclei, where they remain antigenically reactive f...

During the late phase of simian virus 40 infections of CV1 cells, the relative ratios of the spliced to the unspliced RNA molecules of the 19S family were measured. In the cytoplasm, unspliced 19S RNA represented between 1 and 2% of spliced 19S RNA. This ratio could be altered by the use of different methods of RNA extraction such that unspliced RNA was observed at 10 to 20% of spliced RNA. The...

Fitch, Mark D., and Sharon E. Fleming. Metabolism of short-chain fatty acids by rat colonic mucosa in vivo. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G31–G40, 1999.—To determine the influence of substrate concentration and substrate interactions on short-chain fatty acid metabolism in vivo, a surgical procedure was established. Rats were surgically operated to cannulate a 5-cm segm...

Menezes, John, Christian Hierholzer, Simon C. Watkins, Valerie Lyons, Andrew B. Peitzman, Timothy R. Billiar, David J. Tweardy, and Brian G. Harbrecht. A novel nitric oxide scavenger decreases liver injury and improves survival after hemorrhagic shock. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G144–G151, 1999.—We tested the ability of a nitric oxide (NO) scavenger to reduce tissue ...

however, a new restriction, banning a specific late-term abortion procedure, was the focal point in numerous states. And for the first time in seven years, no measures were enacted to safeguard abortion clinics and providers from harassment and acts of violence. The drop in state-level activity in this area is likely attributable to a decease in the number of reported incidents against abortion...

NAZZARENO BALLATORI,1 DAVID N. HAGER,2 SURAJIT NUNDY,3 DAVID S. MILLER,4 AND JAMES L. BOYER5,6 1Department of Environmental Medicine, University of Rochester School of Medicine, Rochester 14642; 2Cornell University Medical College, New York, New York 10021; 3Washington University School of Medicine, St. Louis, Missouri 63110; 4Laboratory of Pharmacology and Chemistry, NIEHS/NIH, Research Triang...

Horowitz, Jeffrey F., Renata J. Braudy, Wade H. Martin III, and Samuel Klein. Endurance exercise training does not alter lipolytic or adipose tissue blood flow sensitivity to epinephrine. Am. J. Physiol. 277 (Endocrinol. Metab. 40): E325–E331, 1999.—We evaluated the relationship between lipolysis and adipose tissue blood flow (ATBF) in response to epinephrine and the effect of endurance exercis...

Zeng, Ningxin, Christoph Athmann, Tao Kang, John H. Walsh, and George Sachs. Role of neuropeptidesensitive L-type Ca21 channels in histamine release in gastric enterochromaffin-like cells. Am. J. Physiol. 277 (Gastrointest. Liver Physiol. 40): G1268–G1280, 1999.—Peptides release histamine from enterochromaffin-like (ECL) cells because of elevation of intracellular Ca21 concentration ([Ca]i) by ...