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White-tailed deer participate in the maintenance of the Ixodes tick life cycle and are reservoirs for some tick-borne infectious agents. Deer may be useful sentinels for tick-transmitted agents, such as ehrlichiae. In order to determine whether white-tailed deer are markers of natural transmission or are reservoirs for the human granulocytic ehrlichiosis (HGE) agent, we performed indirect immun...
Municipal solid waste (MSW) incineration is a common and effective practice to reduce the volume of solid waste in urban areas. However, the byproduct of this process is a fly ash (IFA), which contains large quantities of toxic contaminants. The purpose of this research study was to analyze the chemical, physical and mechanical behaviors resulting from the gradual introduction of IFA to an alka...
Scrub typhus is transmitted by trombiculid mites and is endemic to East and Southeast Asia and Northern Australia. The clinical syndrome classically consists of a fever, rash, and eschar, but scrub typhus also commonly presents as an undifferentiated fever that requires laboratory confirmation of the diagnosis, usually by indirect fluorescent antibody (IFA) assay. We discuss the limitations of ...
there are few reports concerning interferon induced myocardial dysfunction. a known case of multiple myeloma who was receiving interferon alpha (ifa) for 14 months was brought to the emergency room in frank: pulmonary edema. after treatment of heart failure and discontinuation of interferon alpha, he remained asymptomatic thereafter. another case of chronic active hepatitis on ifa complained of...
A total of 349 human serum samples were examined for anti-Toxoplasma antibodies by the Murex Single Use Diagnostic System (SUDS) qualitative screening test, indirect hemagglutination assay (IHA), and indirect immunofluorescence assay (IFA). Concordant results with SUDS and IHA were obtained for 91.9% of serum samples; 8.9% were SUDS+ and IHA-; none were SUDS- and IHA+. Comparison of the SUDS as...
A total of 349 human serum samples were examined for anti-Toxoplasma antibodies by the Murex Single Use Diagnostic System (SUDS) qualitative screening test, indirect hemagglutination assay (IHA), and indirect immunofluorescence assay (IFA). Concordant results with SUDS and IHA were obtained for 91.9% of serum samples; 8.9% were SUDS+ and IHA-; none were SUDSand IHA+. Comparison of the SUDS assa...
The Incremental Focus of Attention (IFA) framework provides a structure in which diierent algorithms for visual motion tracking can be assembled into a single system which tracks an object with the best combination of precision, speed, and robustness. IFA puts diierent tracking algorithms in a hierarchy based on speed and precision and supplies a means for determining which algorithm should per...
Reactivity of human sera to different strains of granulocytic ehrlichiae in immunodiagnostic assays.
Sera from 35 patients diagnosed with human granulocytic ehrlichiosis in Connecticut were tested by indirect IFA staining methods with 5 strains of Ehrlichia equi or the human granulocytic ehrlichiosis agent to assess the suitability of different strains in laboratory analyses. Antigens included horse-derived infected neutrophils (MRK and BDS strains) and human isolates cultured in human promyel...
Monoclonal antibodies were produced against parainfluenza virus type 3 (PI-3) and used to identify PI-3 clinical isolates in cell culture and PI-3 antigen in cells obtained from nasopharyngeal (NP) washes of patients. Two (2E9 and 4G5) of the three monoclonal antibodies characterized reacted by immunoblotting with a 67,000-dalton PI-3 protein, and one antibody (4E5) reacted with two viral prote...
In Independent Factor Analysis (IFA), latent components (or sources) are recovered from only their linear observed mixtures. Both the mixing process and the source densities (that are assumed to be generated according to mixtures of Gaussians) are learned from observed data. This paper investigates the possibility of estimating the IFA model in its noiseless setting when two kinds of prior info...
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