Dark noise, light-induced noise and responses to brief flashes of light were recorded in the membrane current of isolated rods from larval tiger salamander retina before and after bleaching most of the native visual pigment, which mainly has the 11-cis-3,4-dehydroretinal (A2) chromophore, and regenerating with the 11-cis-retinal (A1) chromophore in the same isolated rods. The purpose was to tes...

Recent high throughput sequencing analysis has revealed that the TCRβ repertoire is largely different between CD8(+) and CD4(+) T cells. Here, we show that the transduction of SIG35α, the public chain-centric HLA-A*02:01(A2)/MART127-35 TCRα hemichain, conferred A2/MART127-35 reactivity to a substantial subset of both CD8(+) and CD4(+) T cells regardless of their HLA-A2 positivity. T cells indiv...

The HLA-A2-specific mouse monoclonal antibody BB7.2 plus complement has been used to immunoselect variant clones of the lymphoblastoid cell line T5-1 (HLA-A1, -A2, -B8, and -B27). Members of one class of variant clones appear to express cell surface HLA-A2 molecules that display reduced reactivity with the selecting antibody, but normal or near normal reactivities with some other HLA-A2-specifi...

Accurate and precise hemoglobin separation and the quantitation of Hb A2 and Hb F are essential for the diagnosis of the thalassemias and hemoglobinopathies. Presented in this study is the validation of the the Hb A2 assay of the HbGold analyzer, a fully automated liquid chromatography system for hemoglobin separation and quantitation. Variability of Hb A2 quantitation was quite low; the CV's o...

Suppose a, b, c, and d are rational numbers such that a2, b2, c2, and d2 form an arithmetic progression: the differences b2−a2, c2−b2, and d2−c2 are equal. One possibility is that the arithmetic progression is constant: a2, a2, a2, a2. Are there arithmetic progressions of four rational squares which are not constant? This question was first raised by Fermat in 1640. There are no such progressio...

While the factorization assumption works well for many two-body nonleptonic B meson decay modes, the recent measurement of B̄ → D(∗)0M0 with M = π, ρ and ω shows large deviation from this assumption. We analyze the B → D(∗)M decays in the perturbative QCD approach based on kT factorization theorem, in which both factorizable and nonfactorizable contributions can be calculated in the same framewo...

Richman, Jeremy G., and John W. Regan. a2-Adrenergic receptors increase cell migration and decrease F-actin labeling in rat aortic smooth muscle cells. Am. J. Physiol. 274 (Cell Physiol. 43): C654–C662, 1998.—Vascular wound healing and such pathologies as atherosclerosis and restenosis are characterized by migration and proliferation of the smooth muscle cells of the media after denudation of t...

Adenosine diphosphate (ADP) is a platelet agonist that causes platelet shape change and aggregation as well as generation of thromboxane A2, another platelet agonist, through its effects on P2Y1, P2Y12, and P2X1 receptors. It is now reported that both 2-propylthio-D-dichloromethylene adenosine 5 -triphosphate (AR-C67085), a P2Y12 receptor–selective antagonist, and adenosine-2 -phosphate-5 -phos...

Synthesis of progeny DNA genomes in cells infected by human subgroup C adenoviruses leads to several changes in viral gene expression. These changes include transcription from previously silent, late promoters, such as the IV(a2) promoter, and a large increase in the efficiency of major-late (ML) transcription. Some of these changes appear to take place sequentially, because the product of the ...

BACKGROUND As a potent CD8(+) T cell activator, peptide vaccine has found its way in vaccine development against intracellular infections and cancer, but not against leishmaniasis. The first step toward a peptide vaccine is epitope mapping of different proteins according to the most frequent HLA types in a population. METHODS AND FINDINGS Six Leishmania (L.) major-related candidate antigens (...