نتایج جستجو برای: ترانسکریپتوم و rna seq

تعداد نتایج: 1015057  

2014
Matthew D. Wilkerson Christopher R. Cabanski Wei Sun Katherine A. Hoadley Vonn Walter Lisle E. Mose Melissa A. Troester Peter S. Hammerman Joel S. Parker Charles M. Perou D. Neil Hayes

Identifying somatic mutations is critical for cancer genome characterization and for prioritizing patient treatment. DNA whole exome sequencing (DNA-WES) is currently the most popular technology; however, this yields low sensitivity in low purity tumors. RNA sequencing (RNA-seq) covers the expressed exome with depth proportional to expression. We hypothesized that integrating DNA-WES and RNA-se...

Journal: : 2021

Bioinformatics Study of mtDNA Genes in Two Subspecies Holstein (Bos taurus) and Cholistani indicus) Cattle Using RNA-Seq Data

Journal: :Briefings in functional genomics 2012
Paul L Auer Sanvesh Srivastava R W Doerge

RNA-sequencing (RNA-seq) technologies have not only pushed the boundaries of science, but also pushed the computational and analytic capacities of many laboratories. With respect to mapping and quantifying transcriptomes, RNA-seq has certainly established itself as the approach of choice. However, as the complexities of experiments continue to grow, there is still no standard practice that allo...

2013
V. G. Cheung R. R. Nayak I. X. Wang S. Elwyn R. S. Spielman S. B. Montgomery

Title RNA-seq count data employed in the vignette of the tweeDEseq package Description RNA-seq count data from Pickrell et al. (2010) employed to illustrate the use of the Poisson-Tweedie family of distributions with the tweeDEseq package.

Journal: :Briefings in functional genomics 2015
Francesca Finotello Barbara Di Camillo

RNA-seq is a methodology for RNA profiling based on next-generation sequencing that enables to measure and compare gene expression patterns at unprecedented resolution. Although the appealing features of this technique have promoted its application to a wide panel of transcriptomics studies, the fast-evolving nature of experimental protocols and computational tools challenges the definition of ...

2014
David Loughrey Kyle E. Watters Alexander H. Settle Julius B. Lucks

RNA structure is a primary determinant of its function, and methods that merge chemical probing with next generation sequencing have created breakthroughs in the throughput and scale of RNA structure characterization. However, little work has been done to examine the effects of library preparation and sequencing on the measured chemical probe reactivities that encode RNA structural information....

Journal: :Nature Reviews Genetics 2013

2017
Yong-Wei Yan Bin Zou Ting Zhu Wael N Hozzein Zhe-Xue Quan

RNA-seq-based SSU (small subunit) rRNA (ribosomal RNA) analysis has provided a better understanding of potentially active microbial community within environments. However, for RNA-seq library construction, high quantities of purified RNA are typically required. We propose a modified RNA-seq method for SSU rRNA-based microbial community analysis that depends on the direct ligation of a 5' adapto...

Journal: :Current Protocols in Molecular Biology 2018

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