نتایج جستجو برای: انگشت نگاری dgge

تعداد نتایج: 7753  

Journal: :Environmental microbiology 2007
Rui Zhang Markus G Weinbauer Pei-Yuan Qian

To gain a better understanding of the interactions among bacteria, viruses and flagellates in coastal marine ecosystems, we investigated the effect of viral lysis and protistan bacterivory on bacterial abundance, production and diversity [determined by 16S rRNA gene polymerase chain reaction (PCR) and denaturing gradient gel electrophoresis (DGGE)] in three coastal marine sites with different n...

Journal: :Journal of forensic sciences 2004
Kazumasa Sekiguchi Hajime Sato Kentaro Kasai

A denaturing gradient gel electrophoresis (DGGE) assay was used to detect mitochondrial DNA (mtDNA) sequence heteroplasmy in 160 hairs from each of three individuals. The HV1 and HV2 heteroplasmic positions were then identified by sequencing. In several hairs, the heteroplasmic position was not evident by sequencing and dHPLC separation of the homoduplex/heteroduplex species was carried out wit...

Journal: :Biological & pharmaceutical bulletin 2013
Nobuyasu Yamaguchi Takahiro Nishiguchi Fuangfa Utrarachkij Orasa Suthienkul Masao Nasu

In Southeast Asian countries, industrialization and urbanization is occurring rapidly, and water pollution in rivers and canals poses serious problems in some areas, especially in cities. Excess inflow of domestic, agricultural, and industrial wastewater to freshwater environments disturbs the aquatic microbial ecosystem, which can further pollute water by inhibiting biodegradation of pollutant...

2012
Jun Li Jiaojiao Xin Liyuan Zhang Longyan Jiang Hongcui Cao Lanjuan Li

OBJECTIVE To establish a rapid detection method for identifying rpoB mutations associated with rifampin (RIF) resistance in sputum specimens. METHODS We detected rpoB mutations directly in 90 sputum specimens collected from suspected tuberculosis patients using PCR-based denaturing gradient gel electrophoresis (DGGE) and compared these results with those obtained by rpoB sequencing and conven...

Journal: :FEMS microbiology ecology 2006
Naoise Nunan Brajesh Singh Eileen Reid Brian Ord Artemis Papert Julie Squires Jim I Prosser Ron E Wheatley Jim McNicol Peter Millard

Soil microbial communities play an important role in nutrient cycling and nutrient availability, especially in unimproved soils. In grazed pastures, sheep urine causes local changes in nutrient concentration which may be a source of heterogeneity in microbial community structure. In the present study, we investigated the effects of synthetic urine on soil microbial community structure, using ph...

Journal: :Applied and environmental microbiology 2001
R M Satokari E E Vaughan A D Akkermans M Saarela W M de Vos

We describe the development and validation of a method for the qualitative analysis of complex bifidobacterial communities based on PCR and denaturing gradient gel electrophoresis (DGGE). Bifidobacterium genus-specific primers were used to amplify an approximately 520-bp fragment from the 16S ribosomal DNA (rDNA), and the fragments were separated in a sequence-specific manner in DGGE. PCR produ...

Journal: :Journal of applied microbiology 2008
H Albano I Henriques A Correia T Hogg P Teixeira

AIMS This study evaluates the microbial ecology of 'Alheira' by traditional microbiological analysis and a PCR-denaturing gradient gel electrophoresis (DGGE) protocol. METHODS AND RESULTS Total microbial DNA from 'Alheiras' was extracted directly from the products and subjected to PCR using Eubacterial primers for 16S rDNA. The amplicons were separated by DGGE. The results demonstrated that d...

Journal: :Applied and environmental microbiology 2004
Tatsunori Nakagawa Jun-Ichiro Ishibashi Akihiko Maruyama Toshiro Yamanaka Yusuke Morimoto Hiroyuki Kimura Tetsuro Urabe Manabu Fukui

This study describes the occurrence of unique dissimilatory sulfite reductase (DSR) genes at a depth of 1,380 m from the deep-sea hydrothermal vent field at the Suiyo Seamount, Izu-Bonin Arc, Western Pacific, Japan. The DSR genes were obtained from microbes that grew in a catheter-type in situ growth chamber deployed for 3 days on a vent and from the effluent water of drilled holes at 5 degrees...

Journal: :Applied and environmental microbiology 2001
J Walter C Hertel G W Tannock C M Lis K Munro W P Hammes

Denaturing gradient gel electrophoresis (DGGE) of DNA fragments generated by PCR with 16S ribosomal DNA-targeted group-specific primers was used to detect lactic acid bacteria (LAB) of the genera Lactobacillus, Pediococcus, Leuconostoc, and Weissella in human feces. Analysis of fecal samples of four subjects revealed individual profiles of DNA fragments originating not only from species that ha...

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