The micropipet delivery-substrate collection (MD-SC) mode of scanning electrochemical microscopy (SECM) is demonstrated. This new mode is intended for the study and imaging of electrochemical as well as electrocatalytic reactions of neutral species that cannot be generated electrochemically. The spontaneous transfer of the analyte from an organic solvent across an interface between two immiscib...

Regulation of translating ribosomes is a major component of gene expression control network. In Escherichia coli, ribosome stalling by the C-terminal arrest sequence of SecM regulates the SecA-dependent secretion pathway. Previous studies reported many residues of SecM peptide and ribosome exit tunnel are critical for stalling. However, the underlying molecular mechanism is still not clear at t...

Scanning electrochemical microscopy (SECM) and copper staining are combined to achieve visualisation of proteins on surfaces. Proteins are adsorbed on a polyvinylidene fluoride (PVDF) membrane or on a polyethylene terephthalate (PET) surface and stained using a standard protocol involving copper salts. Salts are then reduced to copper and detected by SECM with ferrocene methanol as a redox medi...

In this work, composite Ni–P-multiwalled carbon nanotube films were produced by electroless deposition. The main goal was to investigate the influence of multiwalled loading on local electrochemical behavior films, as probed scanning microscopy (SECM). coatings also characterized with respect their crystalline structure, surface, and cross-section morphologies. Adhesion strength examined scratc...

Electrodeposition is an important approach that can produce functional compound materials by assembling multiple species at the electrode surface. However, a fundamental understanding of the electrodeposition mechanism has been limited by its complexity and is often gained only through ex situ studies of deposited materials. Here we report on the application of scanning electrochemical microsco...

Here we report on the unprecedentedly high resolution imaging of ion transport through single nanopores by scanning electrochemical microscopy (SECM). The quantitative SECM image of single nanopores allows for the determination of their structural properties, including their density, shape, and size, which are essential for understanding the permeability of the entire nanoporous membrane. Nanos...

Porcine urinary bladder is one of the most used organs to fabricate extracellular matrix (ECM) hydrogel. Although there are two different ECM types inside a bladder, i.e., (UBM) and subtype (sECM), studies have only employed UBM for hydrogel fabrication, overlooked potential use sECM. In another aspect, delamination from bladders time-consuming process; consequently, whole (WB) will likely incr...