The detection of protein lysine acylations remains a challenge due to lack of specific antibodies for acylations with various chain lengths. This problem can be addressed by metabolic labeling techniques using carboxylates with reactive functionalities. Subsequent chemoselective reactions with a complementary moiety connected to a detection tag enable the visualization and quantification of the...

By SANFORD LEIKIN AND SAMUEL P. BESSMAN D ISODIUM ETHYLEXEDIAMINE TETRAACETATE (EDTA) has beets known for some time to prolong coagulation in vitro.’3 During the course of treatment of seven patiemits for lead ititoxication with disodium calcium EDTA, we noted mio hemorrhagic mamiifestations.4’ However, Wishimisky6 described hypoprothrombimsemia appearing its a patiemit treated with disodium ca...

By SANFORD LEIKIN AND SAMUEL P. BESSMAN D ISODIUM ETHYLEXEDIAMINE TETRAACETATE (EDTA) has beets known for some time to prolong coagulation in vitro.’3 During the course of treatment of seven patiemits for lead ititoxication with disodium calcium EDTA, we noted mio hemorrhagic mamiifestations.4’ However, Wishimisky6 described hypoprothrombimsemia appearing its a patiemit treated with disodium ca...

This study evaluated the effect of EDTA and lysozyme on the antibacterial activities of activated ovotransferrin against 5 strains of Listeria monocytogenes. First, a disc test was performed to screen the concentrations of EDTA or lysozyme that showed antibacterial activities in ovotransferrin (O) or ovotransferrin in 100 mM NaHCO3 (OS) solution. Turbidity and viability tests were conducted usi...

We tested the hypothesis that controlled deficit irrigation (CDI) of the fast growing, salinity resistant Eucalyptus camaldulensis tree with timely EDTA application can enhance sediment clean-up while minimizing leaching of metal complexes. 220-L lysimeters containing a sand-metal-polluted sludge mixture. Established saplings were irrigated with tap or desalinized (RO) water with/without 4-time...

Chelating and ion-exchange N-functionalized mesoporous silicas (SBA-15) as selective adsorbents for removal of heavy metals were synthesized using template method. Fourier transform infrared spectroscopy, TEM analysis, N2 adsorption/desorption isotherms titration analysis confirmed successful functionalisation the tri-sodium salt N-(triethoxysilylpropyl)ethylenediaminetriacetic acid (EDTA), pro...

A facile and reversible method for assembling and disassembling gold nanorods (GNRs) using a common chelating agent, ethylenediaminetetraacetic acid (EDTA), is reported. Assembly was induced by the electrostatic interaction between the cetyltrimethylammonium bromide (CTAB) bilayer present on GNRs and EDTA. At lower concentrations of EDTA, end-to-end assembled chains were formed. At higher conce...

This in vitro study evaluated (1) the dissolution of bovine pulp tissue in solutions consisting of varying NaOCl concentrations and combined with EDTA; and (2) the pH of these solutions before and after the experiment. The independent variables were the concentration and the volume of the solution. Thirty bovine pulps were divided in equal fragments, resulting in 90 fragments of pulp tissue. Ea...

A pot study was used to examine the effects of amendments such as EDTA and elemental sulfur on the growth potential, gas exchange features, uptake and mobilization of Pb by wheat (Triticum aestivum L.) in two texturally different contaminated soils at three levels of EDTA (2, 4, 8 mmol kg(-1) dry soil) and two levels of elemental sulfur (100, 200 mmol kg(-1) dry soil). EDTA resulted in more sol...

Cultures of chick heart fibroblasts were perfused with the chelating agent ethylenediaminetetraacetic acid (EDTA). Cellular responses were observed under phase optics and recorded by time-lapse cinemicrography. In interphasic fibroblasts, EDTA induces cellular contraction followed by continuous protrusion and retraction of ectoplasmic blebs ("surface bubbling"), formation of motile vermiform pr...