In one of 30 transgenic tobacco (Nicotiana tabacum) plants, the expression of an introduced beta-glucuronidase (GUS) gene driven by the cauliflower mosaic virus 35S promoter, was found to be repressed as the plant matured, whereas the endogenous GUS activity was unaffected. Plants grown from seeds or regenerated from leaf discs derived from this plant showed a similar temporal pattern of expres...

To study whether DNA pairing that influences gene expression can take place in somatic plant cells, a system designed to mimic transvection was established in transgenic tobacco. Pairing was evaluated by testing whether an enhancerless GUS gene on one allele could be activated in trans by an enhancer on the second allele. The required heteroalleles were obtained at four genomic locations using ...

A stable Agrobacterium-mediated transformation system was established using bialaphos as the selective agent in soybeans [Glycine max (L.) Merr.]. The cotyledonary node explants of the soybean cultivar ‘NY-1001’ were inoculated with the Agrobacterium tumefaciens strain EHA105, harboring the vector pCAMBIA3301 containing the gus gene as the reporter gene and the bar gene conferring bialaphos res...

Transient expression profiles for several chimeric beta-glucuronidase (GUS) gene constructs were determined in tissues (young leaves, mature leaves and roots) of creeping bentgrass (Agrostis palustris, cv. Penn A4) following microprojectile bombardment. The constructs analyzed consisted of the uidA (GUS) reporter gene driven by four different promoters (ubiquitin 3-potato, ubiquitin corn, ubiqu...

In this letter, we study the deployment of Unmanned Aerial Vehicle mounted Base Stations (UAV-BSs) in multi-UAV cellular networks. We model problem as a user satisfaction maximization problem, that is, maximizing proportion served ground users (GUs) meet given minimum data rate requirement. propose an interference-aware (IAD) algorithm for serving arbitrarily distributed outdoor GUs. The propos...

Inserts bearing the coding sequences of NPT II and beta-glucuronidase (GUS) were placed between the nuclear inclusion b (NIb) and coat protein (CP) domains of the wheat streak mosaic virus (WSMV) polyprotein ORF. The WSMV NIb-CP junction containing the nuclear inclusion a (NIa) protease cleavage site was duplicated, permitting excision of foreign protein domains from the viral polyprotein. Whea...

Background The IL-23p19 inhibitor guselkumab (GUS) has shown robust efficacy vs placebo (PBO) in phase 3 DISCOVER-1 and -2 studies of adults with active psoriatic arthritis (PsA) [1,2] , continuous overall clinical improvement observed through 2 years DISCOVER-2 [3] . GUS previously been to significantly reduce levels inflammatory biomarkers associated PsA over 24 weeks [4] Objectives To assess...

Gossypium hirsutum cellulose synthase catalytic subunit 4 (GhCesA4) plays an important role in cellulose biosynthesis during cotton fiber development. The transcript levels of GhCesA4 are significantly up-regulated as secondary cell wall cellulose is produced in developing cotton fibers. To understand the molecular mechanisms involved in transcriptional regulation of GhCesA4, β-glucuronidase (G...

p-Chlorophenoxyisobutyric acid (PCIB) is known as a putative antiauxin and is widely used to inhibit auxin action, although the mechanism of PCIB-mediated inhibition of auxin action is not characterized very well at the molecular level. In the present work, we showed that PCIB inhibited BA::beta-glucuronidase (GUS) expression induced by indole-3-acetic acid (IAA), 2,4-dichlorophenoxyacetic acid...

Article history: Received on: 16/05/2016 Revised on: 10/06/2016 Accepted on: 20/06/2016 Available online: 05/11/2016 In plants, pollen tube germination occurs widely in flowering plants. In Arabidopsis thaliana, it has been reported ARABINOGALACTAN PROTEIN 1 (AGP1) plays an important role in pollen tube germination. The expression of arabinogalactan protein in Arabidopsis pollen tubes has been ...