Complex formation of the glutathione peroxidase mimics 2,2′-ditelluro-bridged β-cyclodextrin (1) and 2,2′-diseleno-bridged β-cyclodextrin (2), with S-substituted dinitrophenyl glutathione (3) were determined by ultraviolet-visible (UV-Vis) absorption spectroscopy in phosphate buffer (pH 7.4) and H-NMR spectroscopy. Molecular mechanics (MM2) modeling calculations were used to deduce a three-dime...

Baicalin (BC) has been used for cancer therapy due to its multiple effects as an anti-cancer drug. However, the effective delivery of this molecule to targeted cells is difficult. Gold nanoparticles (AuNPs) conjugated with thiolated beta cyclodextrin (AuNP-S-β-CD) were used as a delivery vector in this study. Cell viability tests were evaluated by cell counting kit-8 (CCK) and live/dead cell as...

The preservation of Bacillus firmus strain 37 cells by lyophilization was evaluated and response surface methodology (RSM) was used to optimize the β-cyclodextrin (β-CD) production by cells immobilized on loofa sponge. Interactions were studied with the variables temperature, pH and dextrin concentration using a central composite design (CCD). Immobilization time influence on β-CD production wa...

Objective: To develop a simple and cost effective chiral HPLC method for the separation of citalopram (CIT) enantiomers using chiral mobile phase additives (CMPAs). Methods: Sulfated beta cyclodextrin (S-β-CD) was synthesized in our laboratory and was evaluated as a CMPA. The parameters affecting the resolution were optimized. CIT enantiomers were resolved on an achiral Kromasil C8 column (150 ...

Isotretinoin (13-cis-Retinoic acid) is a poor water soluble drug, commonly used in the treatment of severe cases of acne. The aim of this project is to formulate isotretinoin in form of tablet and enhance its solubility and dissolution rate; by the use of formulations containing either surfactant (SLS and Tween20) or HP-β-CD. The critical micelle concentration for each surfactant was determined...

Objectives: The objective of this endeavour is to prepare βcyclodextrin (β-CD) inclusion complex with sulfonamide (SAP) using the co-evaporation technique and screened its effect on in-vitro antimicrobial property against some Gram-positive and Gramnegative bacteria. Methods: A new sulfonamide SAP (4-amino-N-(1, 5-dimethyl-3oxo-2-phenyl-2, 3-dihydro-1H-pyrazol-4-yl) benzene sulfonamide) 4 has b...

Scarless wound healing is ideal for patients suffering from soft tissue defects. In this study, we prepared a novel wet dressing (β-CD-ic-CUR/GC) based on the visible light-cured glycol chitosan (GC) hydrogel and inclusion complex between beta-cyclodextrin (β-CD) and curcumin (CUR). We also evaluated its efficacy in the acceleration of wound healing as compared to that of CUR-loaded GC (CUR/GC)...

Allyl isothiocyanate (AITC) is known as an efficient antibacterial agent but it has a very high volatility. Herein, AITC and AITC/β-cyclodextrin (CD)-inclusion complex (IC) incorporated in polyvinyl alcohol (PVA) nanofibers were produced via electrospinning. SEM images elucidated that incorporation of AITC and AITC/β-CD-IC into polymer matrix did not affect the bead-free fiber morphology of PVA...

Circular dichroism (CD) spectroscopy is a widely used technique for the study of protein structure. Numerous algorithms have been developed for the estimation of the secondary structure composition from the CD spectra. These methods often fail to provide acceptable results on α/β-mixed or β-structure-rich proteins. The problem arises from the spectral diversity of β-structures, which has hither...

1. Abstract 2. Introduction 3. Experimental 3.1. Materials 3.2. Sample preparation 3.3. Fluorescence microscopy 3.4. Negative staining TEM 3.5. Cryo-transmission electron microscopy (cryo-TEM) 4. Results and discussion 4.1. From globule to coil: A non-first order transition 4.2. DNA interaction with CTAB and β-CD 4.3. β-CD as a decompacting agent 5. Conclusions and perspectives 6. Acknowledgeme...