Vibrio cholerae causes the potentially lethal disease cholera through the elaboration of the intestinal secretogen cholera toxin. A second toxin of V. cholerae, Zot, decreases intestinal tissue resistance by modifying intercellular tight junctions. In this report, a third toxin of V. cholerae, Ace (accessory cholera enterotoxin), is described. Ace increases short-circuit current in Ussing chamb...

Virulence-associated genes of Vibrio cholerae including O1, O139 and non-O1/non-O139 from an outbreak in Songkhla Province and sporadic cases occurred in Thailand during 1993 - 2002 were investigated. One hundred eighty-five V. cholerae strains were examined for the presence of virulence-associated genes including ctxA, tcpA, zot, toxR, toxS, toxT, and ace by polymerase chain reaction. DNA sequ...

The epithelial response to the opportunistic pathogen Campylobacter concisus is poorly characterised. Here, we assessed the intestinal epithelial responses to two C. concisus strains with different virulence characteristics in Caco-2 cells using RNAseq, and validated a subset of the response using qPCR arrays. C. concisus strains induced distinct response patterns from intestinal epithelial cel...

Zot is an agile and easily extendible bounded model checker, which can be downloaded at http://home.dei.polimi.it/pradella/. The tool supports different logic languages through a multi-layered approach: its core uses PLTL, and on top of it a decidable predicative fragment of TRIO is defined. An interesting feature of Zot is its ability to support different encodings of temporal logic as SAT pro...

Pet World merupakan salah satu jasa pelayanan kebutuhan hewan peliharaan yang berada di Kabupaten Garut, namun pemenuhan tingkat kepuasan pelanggan belum dapat dilakukan secara optimal. Tujuan dari penelitian ini adalah untuk mengetahui layanan berdasarkan pada dimensi kualitas diberikan terhadap serta menghasilkan usulan perbaikan meningkatkan World. Penelitian menggunakan pendekatan Mix Metho...

Information system service quality has been a very important theme in both IS practice and research. User service expectations affect perceived service quality and user satisfaction. The objectives of this research are to i) to explore the relationship between perceived IS service quality and user satisfaction across the three regions of zone of tolerance (ZOT) and ii) to validate the associati...

Background: Zot (Zonula occludens toxin) is one of the secretion toxins of Vibrio cholerae in small intestine that binds to certain receptors in the epithelial cells and causes a change in the structure of tight junction. The purpose of this research is rapid detection of zot enterotoxin gene using PCR. Materials and Methods: The genomic DNA was extracted by DNA isolation kit and gene amplific...

The expectation norm of Information Systems SERVQUAL has been challenged on both conceptual and empirical grounds, drawing into question the instrument’s practical value. To address the criticism that the original IS SERVQUAL’s expectation measure is ambiguous, we test a new set of scales that posits that service expectations exist at two levels that IS customers use as a basis to assess IS ser...

One of the surface energy balance components, the sensible heat flux, can easily be described for modelling and remote sensing purposes by means of the thermal roughness length (zot). This roughness length is usually expressed relative to the roughness length for wind speed (zou): kB−1 = ln(zou/zot). In the past, the kB−1 factor has been taken as a constant having a value of ca. 2 in the case o...

Background: Cholera is a potentially epidemic and life-threatening secretory diarrhoea characterized by voluminous watery stools, often accompanied by vomiting, and resulting in hypovolemic shock and acidosis. It is caused by certain strains of the species Vibrio cholerae which can also cause mild or in apparent infections. The aim of this study is the evaluation of Capsaicin, as a potential in...