egg-laying hens were immunized with the echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. the chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (peg) and ammonium sulphate precipitation method, chloroform extraction and the lithium sulphate precipitation method. these methods were com...

Egg-laying hens were immunized with the Echis carinatus venom and the resulting antibodies were extracted from egg yolk by four different purification methods. The chicken egg yolk antibodies were purified by the water dilution method, polyethylene glycol (PEG) and ammonium sulphate precipitation method, chloroform extraction and the Lithium sulphate precipitation method. These methods were com...

Chicken egg yolk immunoglobulin (IgY) is a superior alternative to mammalian immunoglobulin. However, the practical application of IgY in research, diagnostics, and functional food is limited due to complex or time-consuming purification procedures. The objective of this study was to develop a simple, safe, large-scale separation method for IgY from egg yolk. Egg yolk was diluted with 6-fold de...

The development of an assay for the quantification of chicken immunoglobulin (IgY) and data evaluation is being demonstrated. An enzyme immunoassay was established to perform the quality control of IgY purifications from the egg yolk and to control IgY producing cells in vitro. Each step of the test procedure and possible alternatives were described. The assay was characterized by special test ...

A two-step chromatographic procedure was developed for the isolation and purification of hen IgY antibodies from egg yolk. The antibodies were completely separated from vitellin and lipids by hydrophobic interaction chromatography followed by gel filtration. Almost no residual yolk proteins, no immunoglobulin aggregates, and no antibody fragments could be detected in the final extract. Moreover...

the use of immunoglobulin y (igy) instead of polyclonal antibodies prepared in mammals has been reported recently. these antibodies are found to have better properties in terms of specificity and ease of large scale production. in this study, proteins from egg yolk were extracted with acid and chloroform. the result of cellulose acetate electrophoresis at ph 9 showed some extra bands which were...

Rat immunoglobulin (IgG) was covalently bound to N-(2-hydroxypropyl)methacrylamide (HPMA) copolymers via glycylglycyl spacer. The resultant conjugate, free IgG and HPMA copolymer (containing a low percentage of tyrosinamide to facilitate radiolabelling) were radioiodinated, and their rates of pinocytic uptake, intracellular degradation and exocytic release by rat visceral yolk sacs cultured in ...

The outcome and protective efficacy of maternal antibodies elicited by DNA immunization to the large (L) hepadnavirus envelope protein were studied using the duck hepatitis B virus (DHBV) model. Following genetic immunization of breeding ducks with a DHBV L protein gene-bearing plasmid, specific and highly neutralizing antibodies were transferred from the sera of immunized ducks, via the egg yo...

The potential absorptive role of the yolk sac membrane was evaluated by examining protein and enzyme patterns in embryonic fluids and by comparing the synthetic capacity of the secondary yolk sac, fetal liver and placenta for human chorionic gonadotrophin (HCG) and alpha-fetoprotein (alphaFP). In yolk sac fluid samples, protein electrophoresis showed two main electrophoretic bands with mobiliti...

BACKGROUND There is completely no report about both hen egg anti-lipase immunoglobulin yolk (IgY) and its anti-obesity action. Thus, we tried to isolate and characterize a novel anti-lipase immunoglobulin from hen egg yolk. Moreover, we investigated whether hen egg yolk anti-lipase IgY inhibits pancreatic lipase activity in vitro, and examined its ability to prevent obesity in a murine high fat...