The genetic defects in Prader-Willi syndrome (PWS) and Angelman syndrome (AS) map to 15q11-13. Using microdissection, we have recently isolated several DNA probes for the critical region. Here we report that microclone MN7 detects multiple loci in 15q11-13 and 16p11.2. Eight yeast artificial chromosome (YAC) clones, two genomic phage clones, and two placenta cDNA clones were isolated to analyze...

The recent development of yeast artificial chromosome (YAC) vectors has provided a system for cloning fragments that are over ten times larger than those that can be cloned in more established systems. We have developed a method for the rapid isolation of terminal sequences from YAC clones. The YAC clone is digested with a range of restriction enzymes, a common linker is ligated to the DNA frag...

Background and objectives: Despite the availability of healthcare community services dedicated to cancer survivors, these remain underutilized by young adults living with (YAC; aged 18-39). A workshop was organized in Montréal, Canada, identify needs YAC during their post-treatment transition period explore existing YAC. Methods: We recruited seventeen stakeholders (N = 17), including seven YAC...

A stringent test for imprint control elements is to examine their function at ectopic loci in transgenic experiments. Igf2 and H19 are part of a larger imprinting region and as a first step, we examined these reciprocally imprinted genes in transgenic experiments using a 130 kb YAC clone. After paternal inheritance, H19 was appropriately repressed and Igf2 was expressed, irrespective of copy nu...

The cloning and propagation of large fragments of DNA on yeast artificial chromosomes (YACs) has become a routine and valuable technique in genome analysis. Unfortunately, many YAC clones have been found to undergo rearrangements or deletions during the cloning process. The frequency of transformation-associated alterations and mitotic instability can be reduced in a homologous recombination-de...

We recently demonstrated that the human chromosome 8p21-p12 region encodes a metastasis suppressor gene for rat prostate cancer. The presence of this region suppresses the metastatic ability of rat prostate cancer cells (N. Nihei et al., Genes Chromosomes Cancer, 17: 260-268, 1996). To define further the region harboring the metastasis suppressor gene, a truncated human chromosome 8 containing ...

Gene isolation methods used during positional cloning rely on physical contigs consisting of bacterial artificial chromosomes, P1, or cosmid clones. However, in most instances, the initial framework for physical mapping consists of contigs of yeast artificial chromosome (YACs), large vectors that are suboptimal substrates for gene isolation. Here we report a strategy to identify gene sequences ...

Source and Description: Lambda clone 135 derived from a YAC from the CEPH library, mapping to an interval containing at least part of the Kallmann locus (1). DNA sequences flanking a (C-A)17 repeat of the lambda clone were used to design the polymerase chain reaction (PCR) primers. The polymorphism can be typed using PCR as previously described (2). Polymorphism: Five allelic fragments were res...

The large insert size of yeast artificial chromosomes (YACs; 1) has made them ideal for the analysis of complex genomes. YACs can be restriction mapped with rare cutting enzymes but this does not provide the resolution required to investigate gene structure (such as locating exons and introns). Detailed mapping normally requires sub-cloning into cosmid or phage vectors. Here we describe a metho...