The Bacillus subtilis xylanase A was subjected to site-directed mutagenesis, aimed at changing the interaction with Triticum aestivum xylanase inhibitor, the only wheat endogenous proteinaceous xylanase inhibitor interacting with this xylanase. The published structure of Bacillus circulans XynA was used to target amino acids surrounding the active site cleft of B.subtilis XynA for mutation. Twe...

A new fungus Cladosporium oxysporum GQ-3 producing extracellular xylanase was isolated from decaying agricultural waste and identified based on the morphology and comparison of internal transcribed spacer (ITS) rDNA gene sequence. C. oxysporum produced maximum xylanase activity of 55.92 U/mL with wheat bran as a substrate and NH4Cl as a nitrogen source. Mg(2+) improved C. oxysporum xylanase pro...

Although several xylanases have been studied, only few xylanases from marine micro-organisms have been reported. We report here a novel halotolerant xylanase from marine bacterium Bacillus subtilis cho40 isolated from Chorao island of mandovi estuary Goa, India. Extracellular xylanase was produced by using agricultural residue such as wheat bran as carbon source under solid-state fermentation (...

The most important building block of hemicelluloses is xylan. It is broken down into xylose oligomer residues by Xylanase an enzyme, produced by most organisms, to utilize xylose as primary source of carbon. The Xylanase produced are classified into families, viz 5, 8, 10, 11 and 43 of Glycoside Hydrolases (GH). Xylanase from family GH 11 are monospecific, they consist solely of Xylanase activi...

The xylanase gene from the ruminal bacterium Bacteroides ruminicola 23 is highly expressed in colonic Bacteroides species when carried on plasmid pVAL-RX. In order to stabilize xylanase expression in the absence of antibiotic selection, the xylanase gene was introduced into the chromosome of Bacteroides thetaiotaomicron 5482 by using suicide vector pVAL-7. Xylanase activity in the resulting str...

Xylanase treatment can be an environmentally friendly way to improve the formability and drainability of chemi-mechanical pulp (CMP). Improvements in xylanase treatment efficiency are possible with application of an ultrasonic wave via the cavitation effect. Results showed that the specific surface area (SSA) of the combined treated pulp increased by 14.6% at an ultrasonic treatment of 30 min a...

This study focuses on screening and identification of bacteria, which can produce alkaline xylanase at alkaline pH and high temperature. Bacterial isolates from corncob decaying soil, capable of hydrolyzing xylan were screened. Selected and purified 108 bacterial colonies grown on xylannutrient agar slants were activated and transferred into the fermentation medium. Six highest xylanase produci...

Three experiments were conducted to study the property of xylanase and the effects of xylanase in wheat-based diets on growth performance of broilers, respectively. Experiment 1 was performed in vitro to evaluate the effect of different pH and temperature on xylanase activity, and to evaluate the enzymic stability under different conditions. The results indicated that the optimum temperature an...

In this study, the immobilization of xylanase using a protein-inorganic hybrid nanoflower system, was assessed to improve the enzyme properties. The synthesis of hybrid xylanase nanoflowers was very effective at 4°C for 72 h, using 0.25 mg/mL protein, and efficient immobilization of xylanase was observed with a maximum encapsulation yield and relative activity of 78.5% and 148%, respectively. I...

The present study reports the production of high-level cellulase-free xylanase from Penicillium citrinum isolate HZN13. The variability in xylanase titers was assessed under both solid-state (SSF) and submerged (SmF) fermentation. SSF was initially optimized with different agro-waste residues, among them sweet sorghum bagasse was found to be the best substrate that favored maximum xylanase prod...