نتایج جستجو برای: wide hybridization
تعداد نتایج: 413034 فیلتر نتایج به سال:
background: acute promyelocytic leukemia (apl) is a subclass of acute myeloid leukemia. the chromosomal aberration in 95% of apl cases is t(15; 17) (q22; q21), which prevents cell differentiation. characterization of the underlying molecular lesion is valuable in determining optimal treatment strategy. the goal of this study was to develop a new and powerful flow- fish technique to detect the l...
In situ hybridization (ISH) is a method that uses labeled complementary single strand DNA or RNA to localize specific DNA or RNA sequences in an intact cell or in a fixed tissue section. The main steps of ISH consist of: probe selection, tissue or sample preparation, pre-hybridization treatment, hybridization and washing, detection and control procedure. Probe selection is one of the important ...
پس از شرح مختصری در مورد تولد دانش سیتوژنتیک (1956) و کاربرد آن در پزشکی (1959) به عنوان آزمایش ارزنده ای برای تشخیص بیماریهای ارثی ناشی از ناهنجاریهای کروموزومی، محدودیت دامنه کارآیی آن، محققین را بر آن داشت روش های نواری banding را کشف و به کار گیرند. این روش توانست بسیاری از مشکلات و محدودیت های قبلی را مرتفع نماید ولی باز در مواردی به ویژه در نمونه هایی که امکان کشت بافت مقدور نبود، همچنین...
background: the microarray technology is in needed of cost-effective, low background noise and stable substrates for successful hybridization and analysis. methods: in this research, we developed a three-dimentional stable and mechanically reliable microarray substrates by coating of two polymeric layers on standard microscope glass slides. for fabrication of these substrates, a thin film of ox...
Cyclic voltammetry electrochemical biosensors reported a wide usage and applications for its fast response, able to be miniaturized sensitivity. However, the bulky, expensive laboratory-based readout circuitry made it impossible used in field-based environment. A portable DNA detection using hybridization technique had been design developed this work. It embedded with fabricated FR4 based senso...
Gene flow between closely related species is a frequent phenomenon that is known to play important roles in organismal evolution. Less clear, however, is the importance of hybridization between distant relatives. We present molecular and morphological evidence that support origin of the plant genus Physacanthus via "wide hybridization" between members of two distantly related lineages in the la...
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