Fermentation of lactose in whey permeate directly into ethanol has had only limited commercial success, as the yields and alcohol tolerances of the organisms capable of directly fermenting lactose are low. This study proposes an alternative strategy: treat the permeate with acid to liberate monomeric sugars that are readily fermented into ethanol. We identified optimum hydrolysis conditions tha...

This work aimed at evaluating the influence of enzyme concentration, temperature, and reaction time in the lactose hydrolysis process in milk, cheese whey, and whey permeate, using two commercial β-galactosidases of microbial origins. We used Aspergillus oryzae (at temperatures of 10 and 55°C) and Kluyveromyces lactis (at temperatures of 10 and 37°C) β-galactosidases, both in 3, 6, and 9 U/mL c...

Primary and secondary amines were isolated from whey as their 2,4-dinitrophenyl derivatives, separated by thinlayer partition chromatography, and identified by gas chromatography-mass spectrometry. Examination of raw and pasteurized sweet whey, reconstituted spray dried whey powder, and proteinfree ultrafdtration permeate of sweet and acid wheys revealed the same qualitative distribution of ami...

Kluyveromyces fragilis (CBS 397) is a nonhalophilic yeast which is capable of lactose utilization from whey permeate and high glycerol production under anaerobic growth conditions. However, the optimum yields of glycerol (11.6 mg/ml of whey permeate medium) obtained in this study occurred only in the presence of 1% Na(2)SO(3) as a steering agent. The use of other concentrations of Na(2)SO(3), a...

Previously undescribed oligosaccharides in bovine cheese whey permeate were characterized by a combination of nanoelectrospray Fourier Transform Ion Cyclotron Resonance (nESI-FTICR) mass spectrometry and matrix-assisted laser desorption/ionization Fourier transform ion cyclotron resonance (MALDI-FTICR) mass spectrometry. Oligosaccharide composition was elucidated by collision-induced dissociati...

Continuous production of nisin in laboratory media and whey permeate was investigated using a packed-bed bioreactor. Lactococcus lactis subsp. lactis ATCC 11454 was immobilized by natural attachment to fibre surfaces and entrapment in the void volume within spiral wound fibrous matrix. The inoculated bioreactor was fed M17 medium and effect of pH, temperature, dilution rate, and substrate conce...

The separation of whey proteins from caseins has traditionally been carried out by precipitation of the caseins, either through destabilisation by rennet or by acidification. Microfiltration (MF) is a membrane filtration process that has been investigated for the fractionation of milk proteins [1,2,3]. Microfiltration membranes with molecualr weight cutoff (MWCO) 0.1 μm can be used to separate ...

Aflatoxin M1 (AFM1) is an important mycotoxin frequently found in milk and in dairy products. It is a minor metabolic product of Aspergillus flavus and A parasiticus. However, it occurs in dairy products as a metabolite formed in cows from aflatoxin B1 contained in animal feeds. In cheese production, AFM1 distributes between curd and whey, being present in products derived from cheese whey proc...

The processing of cheese whey pre-concentrated by reverse osmosis is carried out through ultrafiltration in diafiltration mode to produce whey protein concentrates with lower lactose content to be incorporated in beverages. The initial cheese whey protein and lactose contents are 2.13g/100g and 13.22g/100g, respectively.The commercial membranes, GR95PP, supplied by Alfa Laval, Denmark, we...

The purpose of this research was to studythe ability whey protein concentrates (WPC) and permeate produced with ultrafiltration cheese rehydrate. products studied were concentrate witha PDM percentage 80% (WPC-80), permeate, both under the conditions PJSC Dairy “Voronezhsky”.WPC-80 dissolution processes using microscopy. Water-impermeable hydrophobic layers formed at boundary, preventing water ...