Inquiries about various issues relating to Western blot (WB) testing are frequently posted to the Lyme disease discussion groups on the Internet. Among the most commonly asked questions are: What laboratory techniques are used to carry out the assay? What exactly is being measured? What is a “band”? How are the results interpreted? What are the CDC criteria for a “positive” test? Although some ...

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background: cryptosporidium parvum is a parasitic protozoan that functions as important causa­tive agent of diarrhea in human and animals. the host's immune response to surface anti­gens of c. parvum has been previously demonstrated. in this respect, the role of humoral immu­nity in the development of host protective immunity against this protozoon has been well demon­strated. methods: the effe...

objective(s):antibodies against actin, as one of the most widely studied structural and multifunctional housekeeping proteins in eukaryotic cells, are used as internal loading controls in western blot analyses. the aim of this study was to produce polyclonal antibody against a synthetic peptide derived from n-terminal region of β-actin protein to be used as a protein loading control in western ...

background: prostate specific antigen (psa) is an important laboratory marker for diagnosis of prostatic cancer. thus, development of diagnostic tools specific for psa plays an important role in screening, monitoring and early diagnosis of prostate cancer. in this paper, the production and characterization of a panel of murine monoclonal antibodies (mabs) against psa have been presented.  metho...

streptococcus iniae is a major cause of serious bacterial infections in both fish and human beings. capsular polysaccharide (cps) of s. iniae is vital to evade phagocytic clearance of the host and serves as an important protective antigen of s. iniae infection in aquatic animals. the cpsdgene was determined to be highly conservative in capsule polysaccharide operon. prokaryotic expression of th...