Objective Oocyte cryopreservation is an essential part of assisted reproductive technology (ART) which was recently introduced into clinical practice. This study aimed to evaluate the effects of two vitrification systems (Cryotop and Open Pulled Straw, OPS) on mature oocytes gene expressions. MaterialsAndMethods The survival rate of metaphase II (MII) mouse oocytes were assessed after cryoprese...

Vertical thalamocortical afferents give rise to the elementary functional units of sensory cortex, cortical columns. Principles that underlie communication between columns remain however unknown. Here we unravel these by reconstructing in vivo-labeled neurons from all excitatory cell types in the vibrissal part of rat primary somatosensory cortex (vS1). Integrating the morphologies into an exac...

The effects of vitrification on the embryo viability of the Persian sturgeon (Acipenser persicus) were investigated in this study. Vitrificant solutions (VS1-VS6) were prepared by combining propylene glycol (PG) as basic cryoprotectant plus other permeable and non-permeable cryoprotectants in different concentration. Embryos at neurulation stage were selected and incubated in six vitrificant so...

Pantoea americana strain VS1, an extended-spectrum β-lactamase-producing epibiont, was isolated from Magnolia grandiflora in central Florida, USA. Here, we report the de novo whole-genome sequence of this strain, which consists of a total of 191 contigs spanning 5,412,831 bp, with a GC content of 57.3% and comprising 4,836 predicted coding sequences.

Cryopreservation of oocytes would serve as an alternative to overcome the limited availability of dromedary camel oocytes and facilitate improvements in IVP techniques in this species. Our goal was to develop a protocol for the vitrification of camel oocytes at the germinal vesicle (GV) stage using different cryoprotectant combinations: 20% EG and 20% DMSO (VS1), 25% EG plus 25% DMSO (VS2) or 2...

Vermisüspansiyon yenilikçi organik kontrol önlemleri, toprak solucanları ve çeşitli mikroorganizmalar aracılığı ile bitki gelişimini teşvik eden, verimi arttıran, pahalı olmayan, bu nedenle ürün yetiştirme alanlarında tercih edilen yöntemlerden biridir. Bu çalışmada iki farklı vermisüspansiyon uygulamasının biber bitkisi üzerinde beslenen Myzus (N.) persicae Sulzer (Hemiptera: Aphididae)‘nin ge...

Microbially induced calcium carbonate precipitation (MICP) is a phenomenon based on urease activity of halotolerant and alkaliphilic microorganisms that can be used for the soil bioclogging and biocementation in geotechnical engineering. However, enrichment cultures produced from indigenous soil bacteria cannot be used for large-scale MICP because their urease activity decreased with the rate a...

The ability to identify our own body is considered a pivotal marker of self-awareness. Previous research demonstrated that subjects are more efficient in the recognition images representing self rather than others' effectors (self-advantage). Here, we verified whether, at an electrophysiological level, bodily-self modulates change detection responses. In first EEG experiment (discovery sample),...

The cytoarchitectonic subdivision of the neocortex into six layers is often used to describe the organization of the cortical circuitry, sensory-evoked signal flow or cortical functions. However, each layer comprises neuronal cell types that have different genetic, functional and/or structural properties. Here, we reanalyze structural data from some of our recent work in the posterior-medial ba...