Infectious bursal disease virus (IBDV) is a highly contagious pathogen that causes damage in lymphoid organs and remains a threat to the poultry industry worldwide. Currently, subunit vaccines based on VP2 antigen expressed in prokaryotic systems are widely used in clinical settings. However, the immunogenicity of VP2 vaccines is limited because of their inherent defect that the structure of th...

BACKGROUND VP2 of chicken anemia virus (CAV) is a dual-specificity phosphatase required for virus infection, assembly and replication. The functions of the nuclear localization signal (NLS) and nuclear export signal (NES) of VP2 in the cell, however, are poorly understood. Our study identified the presence of a NLS in VP2 and showed that the protein interacted significantly with mini-chromosome...

Porcine parvovirus (PPV) is a major cause of reproductive failure in swine and has caused substantial losses throughout the world. Viral protein 2 (VP2) PPV structural that can self-assemble into virus-like particles (VLP) with hemagglutination (HA) activity. In order to identify essential residues involved mechanism capsid assembly further understand function HA, we analyzed series deletion mu...

Rotaviruses are triple-layered particles that contain four major capsid proteins, VP2, VP4, VP6, and VP7, and two minor proteins, VP1 and VP3. We have cloned each of the rotavirus genes coding for a major capsid protein into the baculovirus expression system and expressed each protein in insect cells. Coexpression of different combinations of the rotavirus major structural proteins resulted in ...

infectious bursal disease (ibd), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (ibdv). to improve the immunogenicity of recombinant ibdv subunitvaccine, an attempt was made to find a new way to prepare ibd vaccine containing glycosylated mvp2antigen. firstly, ibdv mvp2 gene (with a nucleic acid sequence encoding b cell epitope of ibdv(...

Plant viral-based expression system offers an alternative tool for the expression of various target proteins via stable transformation or transient expression in plants. In transient expression strategy, generation of in vitro viral transcripts through complete linearization of the recombinant vector containing the cloned gene is crucial to ensure the success of gene expression in plants. Hence...

Canine parvovirus (CPV) appears to be endemic in almost all populations of wild and domesticated dogs. It causes serious contagious enteric disease. The VP2 protein of CPV is a major capsid protein and plays an important role in the host immune response. In the present study, the recombinant VP2 was expressed in Escherichia coli (E. coli) using the pBAD expression system. Virus DNA from the inf...

Infectious bursal disease (IBD), a highly contagious and devastating disease in young chicken, is causedby infectious bursal disease virus (IBDV). To improve the immunogenicity of recombinant IBDV subunitvaccine, an attempt was made to find a new way to prepare IBD vaccine containing glycosylated mVP2antigen. Firstly, IBDV mVP2 gene (with a nucleic acid sequence encoding B cell epitope of IBDV(...

Fluorescence correlation spectroscopy (FCS) monitors random movements of fluorescent molecules in solution, giving information about the number and the size of for example nano-particles. The canine parvovirus VP2 structural protein as well as N-terminal deletion mutants of VP2 (-14, -23, and -40 amino acids) were fused to the C-terminus of the enhanced green fluorescent protein (EGFP). The pro...

Lactobacillus casei ATCC 393 was selected as an antigen delivery vehicle for 6 mucosal immunization against porcine parvovirus (PPV) infection. A 64kDa fragment of PPV 7 major protective antigen VP2 protein was used as the parvovirus antigen model. A 8 recombinant lactobacilli expressing VP2 protein was constructed with plasmid pPG611.1, 9 where expression and localization of the VP2 protein fr...