BACKGROUND The aim of this study was to investigate the effect of sodium selenite (SS) on reactive oxygen species (ROS) production, total antioxidant capacity (TAC) and glutathione peroxide (GPx) activity of cultured pre-antral follicles derived from vitrified and non-vitrified ovarian tissue. METHODS Immature mouse ovaries were vitrified, and mechanically isolated pre-antral follicles from v...

The objective of this retrospective study was to determine an optimal time point for vitrification of cleavage-stage human embryos. This study included patients who were undergoing day 2 or day 3 vitrified-warmed cleavage-stage embryo transfer at the In Vitro Fertilization (IVF) Programme of the Shanghai First Maternity and Infant Hospital, China, affiliated to the Tongji University School of M...

BACKGROUND Before human MII oocytes are vitrified they are usually denuded from their cumulus cells. In this study we wanted to investigate the effects of an intact corona radiata on the vitrification and fertilization of human oocytes. METHODS The study comprised two different parts. In Part 1, 36 MII stage oocytes, from 6 patients, were randomly assigned into a control group, a group of vit...

Of 125 embryos (category I and II) recovered from 18 does, 25 were maintained intact as control, 25 bisected using a simplified splitting protocol, 25 frozen thawed according to a vitrification method, 25 bisected-vitrified, and 25 vitrified-bisected. The survivability of intact embryos and demiembryos were evaluated after 24 hours of in vitro culture by development ability, morphological asses...

Our aim was to optimize a cryoprotectant treatment for vitrification of immature porcine cumulus-oocyte complexes (COCs). Immature COCs were vitrified either in 35% ethylene glycol (EG), 35% propylene glycol (PG) or a combination of 17.5% EG and 17.5% PG. After warming, the COCs were in vitro matured (IVM), and surviving oocytes were in vitro fertilized (IVF) and cultured. The mean survival rat...

The aim of the present study was to compare fresh and vitrified goat ovarian tissue after autotransplantation and in vitro culture. Adult goats were completely ovariectomised and each ovarian pair was sliced and distributed among six different treatment groups: fresh control, fresh transplant, fresh culture, vitrified control, vitrified transplant and vitrified culture. Follicular morphology, d...

OBJECTIVE This study aimed to evaluate the expression of the genes related to folliculogenesis after vitrification of mouse ovarian tissues using a two-step in vitro culture. MATERIALS AND METHODS In this experimental study, vitrified and non-vitrified ovaries from 7- day old (neonate) female mice were cultured using alpha-Minimum Essential Medium (α-MEM) supplemented with 5% fetal bovine ser...

OBJECTIVES Re-vitrification of embryos immediately after thawing or after a culture period could be used to preserve the extra embryos after embryo transfer. This study aims to clarify the effect of re-vitrification on Bax and Bcl-2 gene expressions of compact and early blastocyst stage embryos. MATERIALS AND METHODS This experimental study was performed on mouse embryos. We collected 8-cell ...

BACKGROUND The mitochondria are an important source of adenosine triphosphate (ATP) production in pre-implantation embryo. Therefore, the objective of this study was to investigate the effect of vitrification and in vitro culture of mouse embryos on their mitochondrial distribution and ATP content. METHODS The embryos at 2-PN, 4-cell and blastocyst stages were collected from the oviduct of st...

BACKGROUND In this study, we evaluated the incidence of apoptosis at the ultrastructural levels and expression of some apoptosis-related genes in vitrified human ovarian tissue just after warming. METHODS Human ovarian tissue biopsies from 23 women after caesarean section were transported to the laboratory within 2 hours, and then they were cut into small pieces. Some pieces were vitrified an...